Source:http://linkedlifedata.com/resource/pubmed/id/16250007
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2005-11-30
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pubmed:databankReference | |
pubmed:abstractText |
RNA interference (RNAi) has become acknowledged as an effective and useful tool to study gene function in diverse groups of cells. We aimed to suppress the expression of the E-cadherin gene during in vitro development of bovine preimplantation embryos using RNAi approach. In this experiment the effect of microinjection of E-cadherin and Oct-4 (as control) double-stranded (ds) RNA on the mRNA and protein expression level of the target E-cadherin gene was investigated. For this, a 496 bp long bovine E-cadherin and 341 bp long Oct-4 dsRNA sample were prepared using in vitro transcription. In vitro produced bovine zygotes were categorized into four treatment groups including those injected with E-cadherin dsRNA, Oct-4 dsRNA, RNase-free water, and uninjected controls. While the injection of E-cadherin dsRNA resulted in the reduction of E-cadherin mRNA and protein levels at the morula and blastocyst stage, the transcript and protein product remained unaffected in the Oct-4 dsRNA, water injected and uninjected control groups. The relative abundance of E-cadherin mRNA in the E-cadherin dsRNA injected morula stage embryos was reduced by 80% compared to the control group (P < 0.05). The Western blot analysis also showed a significant decrease in the E-cadherin protein (119 kDa) in E-cadherin dsRNA injected embryos compared to the other three groups. Microinjection of E-cadherin dsRNA has resulted only 22% blastocyst rate compared to 38%-40% in water injected and uninjected controls. In conclusion, our results indicated the suppression of E-cadherin mRNA and protein has resulted in lower blastocyst rate and the RNAi technology is a promising approach to study the function of genes in early bovine embryogenesis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/DSC2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Desmocollins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-3,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1040-452X
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pubmed:author | |
pubmed:copyrightInfo |
(c) 2005 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
153-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16250007-Animals,
pubmed-meshheading:16250007-Blastocyst,
pubmed-meshheading:16250007-Cadherins,
pubmed-meshheading:16250007-Cattle,
pubmed-meshheading:16250007-Desmocollins,
pubmed-meshheading:16250007-Fertilization in Vitro,
pubmed-meshheading:16250007-Gene Expression Regulation, Developmental,
pubmed-meshheading:16250007-Membrane Glycoproteins,
pubmed-meshheading:16250007-Molecular Sequence Data,
pubmed-meshheading:16250007-Octamer Transcription Factor-3,
pubmed-meshheading:16250007-Phenotype,
pubmed-meshheading:16250007-RNA, Double-Stranded,
pubmed-meshheading:16250007-RNA, Messenger,
pubmed-meshheading:16250007-RNA Interference,
pubmed-meshheading:16250007-Transcription, Genetic,
pubmed-meshheading:16250007-beta Catenin
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pubmed:year |
2006
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pubmed:articleTitle |
Targeted suppression of E-cadherin gene expression in bovine preimplantation embryo by RNA interference technology using double-stranded RNA.
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pubmed:affiliation |
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Endenicher Allee 15, Bonn, Germany.
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pubmed:publicationType |
Journal Article,
In Vitro
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