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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5-7
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pubmed:dateCreated |
2005-10-26
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pubmed:abstractText |
A method was developed for the synthesis of oligonucleotide-cationic peptide conjugates in solution phase by disulfide bond formation. Precipitation was avoided by the easily removable triethylammonium trifluoroacetate (TEATFAc) salt which served at the same time as a buffer of the reaction mixture. The fast and high yielding disulfide bond formation was due to the Npys thio protecting and activating group of Cys. A solution of the free 5-thiol modified oligonucleotide obtained from Poly-Pak purification was used for conjugation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1525-7770
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1059-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16248092-Base Sequence,
pubmed-meshheading:16248092-Buffers,
pubmed-meshheading:16248092-Cations,
pubmed-meshheading:16248092-Chromatography, High Pressure Liquid,
pubmed-meshheading:16248092-Disulfides,
pubmed-meshheading:16248092-Models, Chemical,
pubmed-meshheading:16248092-Molecular Biology,
pubmed-meshheading:16248092-Molecular Sequence Data,
pubmed-meshheading:16248092-Oligonucleotides,
pubmed-meshheading:16248092-Peptides,
pubmed-meshheading:16248092-Salts,
pubmed-meshheading:16248092-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:16248092-Time Factors
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pubmed:year |
2005
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pubmed:articleTitle |
A convenient method for the synthesis of oligonucleotide-cationic peptide conjugates.
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pubmed:affiliation |
Department of Medicinal Chemistry, University of Szeged, Szeged, Dóm tir 8, Hungary.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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