Linked Life Data

16247672

Source:http://linkedlifedata.com/resource/pubmed/id/16247672

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2005-10-25
pubmed:abstractText
Epitopes of a foot-and-mouth disease virus (FMDV) capsid protein VP1 complex and a chimera of 6xHis-tagged cholera toxin B subunit (hCTB) were expressed in Hansenula polymorpha and used together as a mucosal vaccine. Antibody and cytokine responses to VP1-hCTB vaccine and protection against FMDV were evaluated by ELISA and a virus challenge test in mice, respectively. VP1-hCTB directly enhanced the expression of interleukin-5 (IL-5) both in serum and supernatants of cultured spleen cells. After challenging suckling mice with 10(5) FMDV (=50% lethal dosage per mouse) a greater protection was seen after intraperitoneal and intranasal vaccinations than after oral vaccination. In swine immunized with VP1-hCTB, immune responses were achieved after three administrations, and the vaccine protected swine (80%) when challenged with 10(6.5) FMDV (=50% infectious dosage per swine). These results demonstrated the possibility of using CTB as a mucosal adjuvant to elicit protective immune responses against FMDV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0141-5492
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1669-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
A novel mucosal vaccine against foot-and-mouth disease virus induces protection in mice and swine.
pubmed:affiliation
Centre for Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, 13# Zhongguancun Beiyitiao, 100080, Beijing, PR China. songhouhui@yahoo.com.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't