| pubmed-article:16246910 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16246910 | lifeskim:mentions | umls-concept:C0019348 | lld:lifeskim |
| pubmed-article:16246910 | lifeskim:mentions | umls-concept:C0162327 | lld:lifeskim |
| pubmed-article:16246910 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:16246910 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:16246910 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:16246910 | pubmed:dateCreated | 2005-10-25 | lld:pubmed |
| pubmed-article:16246910 | pubmed:abstractText | Herpes simplex virus-1 US11 is a RNA-binding protein with a novel RNA-binding domain. US11 has been reported to exhibit sequence- and conformation-specific RNA-binding, but the sequences and conformations important for binding are not known. US11 has also been described as a double-stranded RNA (dsRNA)-binding protein. To investigate the US11-RNA interaction, we performed in vitro selection of RNA aptamers that bind US11 from a RNA library consisting of >10(14) 80 base sequences which differ in a 30 base randomized region. US11 bound specifically to selected aptamers with an affinity of 70 nM. Analysis of 23 selected sequences revealed a strong consensus sequence. The US11 RNA-binding domain and < or =46 bases of selected RNA containing the consensus sequence were each sufficient for binding. US11 binding protected the consensus motif from hydroxyl radical cleavage. RNase digestions of a selected aptamer revealed regions of both single-stranded RNA and dsRNA. We observed that US11 bound two different dsRNAs in a sequence non-specific manner, but with lower affinity than it bound selected aptamers. The results define a relatively short specific sequence that binds US11 with high affinity and indicate that dsRNA alone does not confer high-affinity binding. | lld:pubmed |
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| pubmed-article:16246910 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16246910 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16246910 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16246910 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16246910 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16246910 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:16246910 | pubmed:author | pubmed-author:EllingtonAndr... | lld:pubmed |
| pubmed-article:16246910 | pubmed:author | pubmed-author:CoenDonald... | lld:pubmed |
| pubmed-article:16246910 | pubmed:author | pubmed-author:CoxJ ColinJC | lld:pubmed |
| pubmed-article:16246910 | pubmed:author | pubmed-author:HogleJames... | lld:pubmed |
| pubmed-article:16246910 | pubmed:author | pubmed-author:BryantKevin... | lld:pubmed |
| pubmed-article:16246910 | pubmed:author | pubmed-author:WangHongmingH | lld:pubmed |
| pubmed-article:16246910 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:16246910 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:16246910 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16246910 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16246910 | pubmed:pagination | 6090-100 | lld:pubmed |
| pubmed-article:16246910 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:16246910 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16246910 | pubmed:articleTitle | Binding of herpes simplex virus-1 US11 to specific RNA sequences. | lld:pubmed |
| pubmed-article:16246910 | pubmed:affiliation | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA. | lld:pubmed |
| pubmed-article:16246910 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16246910 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:16246910 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:2703439 | entrezgene:pubmed | pubmed-article:16246910 | lld:entrezgene |
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