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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
2005-10-25
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pubmed:abstractText |
Herpes simplex virus-1 US11 is a RNA-binding protein with a novel RNA-binding domain. US11 has been reported to exhibit sequence- and conformation-specific RNA-binding, but the sequences and conformations important for binding are not known. US11 has also been described as a double-stranded RNA (dsRNA)-binding protein. To investigate the US11-RNA interaction, we performed in vitro selection of RNA aptamers that bind US11 from a RNA library consisting of >10(14) 80 base sequences which differ in a 30 base randomized region. US11 bound specifically to selected aptamers with an affinity of 70 nM. Analysis of 23 selected sequences revealed a strong consensus sequence. The US11 RNA-binding domain and < or =46 bases of selected RNA containing the consensus sequence were each sufficient for binding. US11 binding protected the consensus motif from hydroxyl radical cleavage. RNase digestions of a selected aptamer revealed regions of both single-stranded RNA and dsRNA. We observed that US11 bound two different dsRNAs in a sequence non-specific manner, but with lower affinity than it bound selected aptamers. The results define a relatively short specific sequence that binds US11 with high affinity and indicate that dsRNA alone does not confer high-affinity binding.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-10882068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-11557339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-11884553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-12052180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-12134014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-12414939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-12477828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-1316472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-14512542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-1656075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-2161949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-7853471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-8383175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-8386887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-8538795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-8627758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-8887567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-9250403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-9603938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-9680120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-9765401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16246910-9847307
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6090-100
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16246910-Base Sequence,
pubmed-meshheading:16246910-Binding Sites,
pubmed-meshheading:16246910-Consensus Sequence,
pubmed-meshheading:16246910-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:16246910-Herpesvirus 1, Human,
pubmed-meshheading:16246910-Hydroxyl Radical,
pubmed-meshheading:16246910-Molecular Sequence Data,
pubmed-meshheading:16246910-Oligonucleotides,
pubmed-meshheading:16246910-Protein Structure, Tertiary,
pubmed-meshheading:16246910-RNA,
pubmed-meshheading:16246910-RNA, Double-Stranded,
pubmed-meshheading:16246910-RNA-Binding Proteins,
pubmed-meshheading:16246910-Ribonucleases,
pubmed-meshheading:16246910-Viral Proteins
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pubmed:year |
2005
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pubmed:articleTitle |
Binding of herpes simplex virus-1 US11 to specific RNA sequences.
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pubmed:affiliation |
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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