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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-10-25
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pubmed:abstractText |
Posttranslational modifications of histones play an essential role in heterochromatin assembly. Whereas the role of Clr4/Suv39h-mediated methylation of histone H3 at lysine 9 (H3K9) in heterochromatin assembly is well studied, the exact function of histone deacetylases (HDACs) in this process is unclear. We show that Clr3, a fission yeast homolog of mammalian class II HDACs, acts in a distinct pathway parallel to RNAi-directed heterochromatin nucleation to recruit Clr4 and mediate H3K9 methylation at the silent mating-type region and centromeres. At the mat locus, Clr3 is recruited at a specific site through a mechanism involving ATF/CREB family proteins. Once recruited, Clr3 spreads across the 20 kb silenced domain that requires its own HDAC activity and heterochromatin proteins including Swi6/HP1. We also demonstrate that Clr3 contributes to heterochromatin maintenance by stabilizing H3K9 trimethylation and by preventing histone modifications associated with active transcription, and that it limits RNA polymerase II accessibility to naturally silenced repeats at heterochromatin domains.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/Clr3 protein, S pombe,
http://linkedlifedata.com/resource/pubmed/chemical/Heterochromatin,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Deacetylases,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Methyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Pcr1 protein, S pombe,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Polymerase II,
http://linkedlifedata.com/resource/pubmed/chemical/Schizosaccharomyces pombe Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Swi6 protein, S pombe,
http://linkedlifedata.com/resource/pubmed/chemical/atf1 protein, S pombe,
http://linkedlifedata.com/resource/pubmed/chemical/clr4 protein, S pombe
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-85
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16246721-Activating Transcription Factor 1,
pubmed-meshheading:16246721-Activating Transcription Factors,
pubmed-meshheading:16246721-Cell Cycle Proteins,
pubmed-meshheading:16246721-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:16246721-Genes, Mating Type, Fungal,
pubmed-meshheading:16246721-Heterochromatin,
pubmed-meshheading:16246721-Histone Deacetylases,
pubmed-meshheading:16246721-Histones,
pubmed-meshheading:16246721-Methylation,
pubmed-meshheading:16246721-Methyltransferases,
pubmed-meshheading:16246721-Phosphoproteins,
pubmed-meshheading:16246721-RNA Interference,
pubmed-meshheading:16246721-RNA Polymerase II,
pubmed-meshheading:16246721-Schizosaccharomyces,
pubmed-meshheading:16246721-Schizosaccharomyces pombe Proteins
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pubmed:year |
2005
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pubmed:articleTitle |
The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeast.
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pubmed:affiliation |
Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural,
Research Support, N.I.H., Intramural
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