Source:http://linkedlifedata.com/resource/pubmed/id/16246612
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2005-11-21
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pubmed:abstractText |
A novel fish-specific apolipoprotein (apo-14 kDa) has been recently cloned from eel and pufferfish. However, its expression pattern has not been elucidated. In this study, EcApo-14 has been screened from hypothalamic cDNA library of male orange-spotted grouper, which shows 62.9%, 51%, 46.9%, 43.2%, and 31.9% identities to Apo-14 of European flounder, pufferfish, Japanese eel, gibel carp, and grass carp, respectively. RT-PCR analysis reveals that this gene is first transcribed in neurula embryos and maintains a relatively stable expression level during the following embryogenesis. EcApo-14 transcripts are at a very high level during embryonic and early larval development in the yolk syncytial layer (YSL), and decrease in YSL and form intense staining in liver at 3 days after hatching. In adult tissues, EcApo-14 is predominantly expressed in liver and brain. The data suggested that EcApo-14 might play an important role in liver and brain morphogenesis and growth.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1096-4959
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
142
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
432-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16246612-Amino Acid Sequence,
pubmed-meshheading:16246612-Animals,
pubmed-meshheading:16246612-Apolipoproteins,
pubmed-meshheading:16246612-Base Sequence,
pubmed-meshheading:16246612-Brain,
pubmed-meshheading:16246612-Cloning, Molecular,
pubmed-meshheading:16246612-DNA, Complementary,
pubmed-meshheading:16246612-Gene Expression Regulation, Developmental,
pubmed-meshheading:16246612-Liver,
pubmed-meshheading:16246612-Male,
pubmed-meshheading:16246612-Molecular Sequence Data,
pubmed-meshheading:16246612-Organ Specificity,
pubmed-meshheading:16246612-Phylogeny,
pubmed-meshheading:16246612-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16246612-Sequence Alignment,
pubmed-meshheading:16246612-Tetraodontiformes
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pubmed:year |
2005
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pubmed:articleTitle |
Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides.
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pubmed:affiliation |
State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan Center for Developmental Biology, Institute of Hydrobiology, Chinese Academy of Sciences, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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