Linked Life Data

1624582

Source:http://linkedlifedata.com/resource/pubmed/id/1624582

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1992-8-7
pubmed:abstractText
A competitive enzyme immunoassay (CEIA) was established and compared with other serological techniques for detecting Coxiella burnetii antibody in camels, goats, and sheep. This technique was evaluated because a conjugated anti-camel immunoglobulin was not available to serve as a direct signal for the demonstration of antigen-antibody reaction. A C. burnetii antibody-positive human serum and a peroxidase-conjugated anti-human immunoglobulin G were used as an indicator system competing against antibody in animal serum or as an indicator of the absence of antibody. Sera were considered antibody positive when the A414 of the test sera plus the competing positive antibody was less than or equal to 50% of the A414 of the negative-control serum plus the competing antibody. Antibody to C. burnetii was repeatedly demonstrated in 66% of camel serum samples (n = 200) by the CEIA. Among 48 camel serum samples, 71% were positive for antibody by CEIA versus 65% by EIA using peroxidase-labeled protein A. The CEIA detected C. burnetii antibody in 63% of sheep serum samples (n = 40) and in 50% of goat serum samples (n = 96), while the indirect fluorescent-antibody technique detected antibody in 38% of sheep and 34% of goat serum samples and the EIA detected antibody in 50% of sheep and 35% of goat serum samples. These data indicate that the CEIA is a reliable and sensitive technique for demonstrating C. burnetii antibody in camels, sheep, and goats.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-1090679, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-2428330, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-2467429, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-3010753, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-3011974, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-3749736, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-4073655, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-4099047, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-4166022, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-4804342, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-5163735, http://linkedlifedata.com/resource/pubmed/commentcorrection/1624582-6339396
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1595-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Evaluation of a competitive enzyme immunoassay for detection of Coxiella burnetii antibody in animal sera.
pubmed:affiliation
Division of Virology, U.S. Naval Medical Research Unit No. 3, Cairo, Egypt.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.