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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1992-8-13
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pubmed:abstractText |
The amino acid sequence of the 13-kDa polypeptide (P116) encoded by the first gene of the trfA operon of IncP plasmid RK2 shows significant similarity to several known single-stranded DNA-binding proteins. We found that unregulated expression of this gene from its natural promoter (trfAp) or induced expression from a strong heterologous promoter (trcp) was sufficient to complement the temperature-sensitive growth phenotype of an Escherichia coli ssb-1 mutant. The RK2 ssb gene is the first example of a plasmid single-stranded DNA-binding protein-encoding gene that is coregulated with replication functions, indicating a possible role in plasmid replication.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-1852017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-1885553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-1987165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2008432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2187181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-221903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-227832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2314282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-232215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2495025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2558866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2651402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2829253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2945079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3003748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3009421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3038679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3045095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-327269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3301414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3318684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-334752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3527040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-383386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3881765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3884590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-3891728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-4044529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-4208284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-4556251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6088948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6186390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6237955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6243621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6244299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6270666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6295879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6308624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6327994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6328430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6346358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6351061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6363409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6374158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6451615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1624472-7043468
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4842-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1624472-Amino Acid Sequence,
pubmed-meshheading:1624472-Bacterial Proteins,
pubmed-meshheading:1624472-Base Sequence,
pubmed-meshheading:1624472-DNA Replication,
pubmed-meshheading:1624472-DNA-Binding Proteins,
pubmed-meshheading:1624472-Escherichia coli,
pubmed-meshheading:1624472-Escherichia coli Proteins,
pubmed-meshheading:1624472-Genetic Complementation Test,
pubmed-meshheading:1624472-Molecular Sequence Data,
pubmed-meshheading:1624472-Mutation,
pubmed-meshheading:1624472-Operon,
pubmed-meshheading:1624472-Plasmids,
pubmed-meshheading:1624472-Promoter Regions, Genetic,
pubmed-meshheading:1624472-Temperature
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pubmed:year |
1992
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pubmed:articleTitle |
The replication initiator operon of promiscuous plasmid RK2 encodes a gene that complements an Escherichia coli mutant defective in single-stranded DNA-binding protein.
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pubmed:affiliation |
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
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