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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2005-10-24
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pubmed:abstractText |
We constructed an expression vector for the coat protein (CP) gene and the 3' untranslated region (3' UTR) of RNA virus (sweet potato feathery mottle virus severe strain (SPFMV-S)) lacking a foreign terminator. Out of seven transgenic tobacco plants, expression of the transgene was observed in six plants. RT-PCR analysis revealed that the transcripts had a poly(A) tail, and in most of them, polyadenylation occurred on the 5' side of the 3' UTR. These results suggest that the viral sequence contains a cryptic polyadenylation signal that permits 3'-end processing of the transcripts.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0916-8451
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2005-8
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pubmed:meshHeading |
pubmed-meshheading:16244460-3' Untranslated Regions,
pubmed-meshheading:16244460-Base Sequence,
pubmed-meshheading:16244460-Capsid Proteins,
pubmed-meshheading:16244460-Molecular Sequence Data,
pubmed-meshheading:16244460-Plants, Genetically Modified,
pubmed-meshheading:16244460-Polyadenylation,
pubmed-meshheading:16244460-RNA, Messenger,
pubmed-meshheading:16244460-RNA, Viral,
pubmed-meshheading:16244460-RNA Viruses,
pubmed-meshheading:16244460-Tobacco
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pubmed:year |
2005
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pubmed:articleTitle |
Cryptic polyadenylation of transcripts of an RNA virus gene introduced into tobacco plants.
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pubmed:affiliation |
National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Bio-oriented Research Organization, Kikuchi, Kumamoto, Japan. tkimura@affrc.go.jp
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pubmed:publicationType |
Journal Article
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