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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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|
pubmed:dateCreated |
2005-12-12
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|
pubmed:abstractText |
Anticancer drug Cyclophosphamide (CY) is metabolized to phosphoramide mustard and acrolein by the hepatic P450 enzymes. GST-Pi is a biochemical feature which occurs in carcinogen induced preneoplastic foci and it plays an important role in the detoxification pathway of acrolein metabolism. Administration of CY induces GST-Pi positive single cells and foci expression in rat liver and these can be considered as precursors of preneoplastic foci leading to hepatocarcinogenesis. The expression of GST-Pi in CY-treated rats on different days of treatment was confirmed by immunohistochemistry, immunoblot, RT-PCR and by ELISA. We also advocate that epigenetic mechanism could be accounted for the GST-Pi induction in the hepatocytes of CY-treated rats.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0300-483X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
217
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
120-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16242832-Acrolein,
pubmed-meshheading:16242832-Animals,
pubmed-meshheading:16242832-Antineoplastic Agents, Alkylating,
pubmed-meshheading:16242832-Cyclophosphamide,
pubmed-meshheading:16242832-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:16242832-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16242832-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:16242832-Glutathione S-Transferase pi,
pubmed-meshheading:16242832-Immunoblotting,
pubmed-meshheading:16242832-Injections, Intraperitoneal,
pubmed-meshheading:16242832-Liver,
pubmed-meshheading:16242832-Male,
pubmed-meshheading:16242832-RNA,
pubmed-meshheading:16242832-Rats,
pubmed-meshheading:16242832-Rats, Wistar,
pubmed-meshheading:16242832-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16242832-Time Factors
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pubmed:year |
2006
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pubmed:articleTitle |
Induction and expression of GST-Pi foci in the liver of Cyclophosphamide-administered rats.
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pubmed:affiliation |
Unit of Biochemistry, Department of Zoology, University of Madras, Life Sciences Building, Guindy Campus, Chennai 600025, Tamil Nadu, India.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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