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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2005-10-24
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pubmed:abstractText |
We designed a fluorescent peptide-magnetic nanoparticle conjugate that images E-selectin expression in mouse xenograft models of Lewis lung carcinoma (LLC) by fluorescence reflectance imaging. It was synthesized by attaching the E-selectin-binding peptide (ESBP; CDSDSDITWDQLWDLMK) to a CLIO(Cy5.5) nanoparticle to yield ESBP-CLIO(Cy5.5). Internalization by activated human umbilical vein endothelial cells (HUVECs) was rapid and mediated by E-selectin, indicated by the lack of uptake of nanoparticles bearing similar numbers of a scrambled peptide (Scram). To demonstrate the specificity of E-selectin targeting to ESBP-CLIO(Cy5.5) in vivo, we coinjected ESBP-CLIO(Cy5.5) and Scram-CLIO(Cy3.5) and demonstrated a high Cy5.5/Cy3.5 fluorescence ratio using the LLC. Histology showed that ESBP-CLIO was associated with tumor cells as well as endothelial cells, but fluorescence-activated cell sorter analysis showed a far less expression of E-selectin on LLC than on HUVECs. Using immunohistochemistry, we demonstrated E-selectin expression in both endothelial cells and cancer cells in human prostate cancer specimens. We conclude that ESBP-CLIO(Cy5.5) is a useful probe for imaging E-selectin associated with the LLC tumor, and that E-selectin is expressed not only on endothelial cells but also on LLC cells and human prostate cancer specimens.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1522-8002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
904-11
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16242073-Animals,
pubmed-meshheading:16242073-Antigens, CD31,
pubmed-meshheading:16242073-Carbocyanines,
pubmed-meshheading:16242073-Carcinoma, Lewis Lung,
pubmed-meshheading:16242073-Cell Line,
pubmed-meshheading:16242073-Cell Line, Tumor,
pubmed-meshheading:16242073-Cell Nucleus,
pubmed-meshheading:16242073-Cell Separation,
pubmed-meshheading:16242073-E-Selectin,
pubmed-meshheading:16242073-Edetic Acid,
pubmed-meshheading:16242073-Endothelial Cells,
pubmed-meshheading:16242073-Endothelium, Vascular,
pubmed-meshheading:16242073-Flow Cytometry,
pubmed-meshheading:16242073-Humans,
pubmed-meshheading:16242073-Immunohistochemistry,
pubmed-meshheading:16242073-Interleukin-1,
pubmed-meshheading:16242073-Male,
pubmed-meshheading:16242073-Mice,
pubmed-meshheading:16242073-Microscopy, Confocal,
pubmed-meshheading:16242073-Microscopy, Fluorescence,
pubmed-meshheading:16242073-Nanostructures,
pubmed-meshheading:16242073-Nanotechnology,
pubmed-meshheading:16242073-Neoplasm Transplantation,
pubmed-meshheading:16242073-Peptides,
pubmed-meshheading:16242073-Prostatic Neoplasms,
pubmed-meshheading:16242073-Sensitivity and Specificity,
pubmed-meshheading:16242073-Substrate Specificity,
pubmed-meshheading:16242073-Time Factors,
pubmed-meshheading:16242073-Umbilical Veins
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pubmed:year |
2005
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pubmed:articleTitle |
Nanoparticles for the optical imaging of tumor E-selectin.
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pubmed:affiliation |
Department of Angiography and Interventional Radiology, Vienna Medical University, Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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