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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2006-6-16
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pubmed:abstractText |
We have characterised the apoptotic defects in cells null for cytochrome c (cyt c-/-). Such cells treated with staurosporine (STS) exhibited translocation to the mitochondria and activation of the proapoptotic signalling molecule Bax but failed to release Smac/DIABLO from these organelles, judged by both confocal microscopy and Western blotting. While reference cells expressing cytochrome c released both it and Smac/DIABLO under a variety of conditions of apoptotic induction, we have never observed release of Smac/DIABLO from cyt c-/- cells. We eliminate the possibility that proteasomal degradation of cytosolically localised Smac/DIABLO is responsible for our failure to visualise the protein outside the mitochondria. Our findings indicate an unanticipated nexus between release of cytochrome c and Smac/DIABLO from mitochondria, previously thought to be a more or less synchronised event early in apoptosis. We suggest that the failure of cyt c-/- cells to release Smac/DIABLO after recruitment of Bax to mitochondria represents an extreme manifestation of some inherent difference in the regulation of release of these two proteins from mitochondria.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1350-9047
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1181-90
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16239929-Animals,
pubmed-meshheading:16239929-Apoptosis,
pubmed-meshheading:16239929-Blotting, Western,
pubmed-meshheading:16239929-Caspases,
pubmed-meshheading:16239929-Cell Line, Tumor,
pubmed-meshheading:16239929-Cytochromes c,
pubmed-meshheading:16239929-Cytosol,
pubmed-meshheading:16239929-Humans,
pubmed-meshheading:16239929-Immunohistochemistry,
pubmed-meshheading:16239929-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:16239929-Mice,
pubmed-meshheading:16239929-Microscopy, Confocal,
pubmed-meshheading:16239929-Mitochondria,
pubmed-meshheading:16239929-Mitochondrial Proteins,
pubmed-meshheading:16239929-NIH 3T3 Cells,
pubmed-meshheading:16239929-Signal Transduction,
pubmed-meshheading:16239929-Staurosporine,
pubmed-meshheading:16239929-bcl-2-Associated X Protein
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pubmed:year |
2006
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pubmed:articleTitle |
Smac/DIABLO is not released from mitochondria during apoptotic signalling in cells deficient in cytochrome c.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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