Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-12-29
pubmed:abstractText
The tetrameric ryanodine receptor calcium release channels (RyRs) are cation-selective channels that have pore architecture similar to that of K+ channels. We recently identified, in close proximity to the selectivity filter motif GGGIG, a conserved lumenal DE motif that has a critical role in RyR ion permeation and selectivity. Here, we substituted three aspartate residues (D4938, D4945, D4953) with asparagine and four glutamate residues (E4942, E4948, E4952, E4955) with glutamine hypothesized to line the cytosolic vestibule of the skeletal muscle RyR (RyR1). Mutant single channel properties were determined using the planar lipid bilayer method. Two mutants (D4938N, D4945N) showed a reduced K+ ion conductance, with D4938N also exhibiting a reduced selectivity for Ca2+ compared to K+. The cytosolic location of D4938 and D4945 was confirmed using the polycation neomycin. Both D4938N and D4945N exhibited an attenuated block by neomycin to a greater extent from the cytosolic than lumenal side. By comparison, charge neutralization of lumenal loop residues (D4899Q, E4900N) eliminated the block from the lumenal but not the cytosolic side. The results suggest that, in addition to negatively charged residues on the lumenal side, rings of four negative charges formed by D4938 and D4945 in the cytosolic vestibule determine RyR ion fluxes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-10097041, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-10473538, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-10551824, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-10920015, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-11427530, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-11916853, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-11916854, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-11964232, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-12037560, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-12208234, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-12270947, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-12438018, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-12565913, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-1279096, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-14557272, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-14593104, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-15226293, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-15336972, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-15361409, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-15454434, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-15863483, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-15908964, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-16084392, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-1642895, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-2298749, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-2443015, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-2459298, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-2539209, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-2725677, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-7744771, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-8133241, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-8274655, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-8345525, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-8384242, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-9085309, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-9234963, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-9525859, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-9788925, http://linkedlifedata.com/resource/pubmed/commentcorrection/16239337-9822655
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
443-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:16239337-Humans, pubmed-meshheading:16239337-Animals, pubmed-meshheading:16239337-Potassium, pubmed-meshheading:16239337-Calcium, pubmed-meshheading:16239337-Glutamine, pubmed-meshheading:16239337-Glutamic Acid, pubmed-meshheading:16239337-Ions, pubmed-meshheading:16239337-Biophysics, pubmed-meshheading:16239337-Mutation, pubmed-meshheading:16239337-Rabbits, pubmed-meshheading:16239337-Asparagine, pubmed-meshheading:16239337-Kinetics, pubmed-meshheading:16239337-Muscle, Skeletal, pubmed-meshheading:16239337-Biophysical Phenomena, pubmed-meshheading:16239337-Neomycin, pubmed-meshheading:16239337-Models, Molecular, pubmed-meshheading:16239337-Cell Line, pubmed-meshheading:16239337-Cytosol, pubmed-meshheading:16239337-Cations, pubmed-meshheading:16239337-Sarcoplasmic Reticulum
More...