Linked Life Data

16237098

Source:http://linkedlifedata.com/resource/pubmed/id/16237098

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2005-10-20
pubmed:abstractText
To understand how vaccines initiate adaptive immune responses, it is necessary to study how they interact with APCs such as dendritic cells (DCs). In this study, we analyzed interactions between recombinant adenovirus (Ad) vectors and mouse DCs. Mouse bone marrow-derived DCs transduced with Ad vectors produced type I IFN, which promoted the maturation of both transduced and bystander DCs. DCs transduced with a vector derived from a chimpanzee Ad serotype (AdC68) produced more type I IFN and matured more efficiently compared with DCs transduced with a vector derived from a human Ad serotype (AdHu5). Both vectors stimulated type I IFN production independently of viral transcription, replication, and TLR signaling. However, each vector induced type I IFN through distinct pathways; whereas AdHu5 vectors required phosphoinositide-3-OH kinase for type I IFN induction, AdC68 vectors did not. Both vectors induced strong transgene product-specific CD8+ T cell responses in wild-type mice. DCs isolated from mice that have a defect in type I IFN signaling failed to undergo full maturation after Ad vaccination, but surprisingly, these mice mounted strong transgene product-specific CD8+ T cell responses. In these mice, we were able to detect a small number of transduced DCs that expressed high levels of costimulatory molecules, and these DCs were able to stimulate transgene product-specific CD8+ T cells. Thus, type I IFN signaling is an important component of Ad-mediated DC maturation but is dispensable during the generation of transgene product-specific CD8+ T cell responses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6032-41
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16237098-Adenoviridae, pubmed-meshheading:16237098-Animals, pubmed-meshheading:16237098-Antigens, CD11c, pubmed-meshheading:16237098-CD8-Positive T-Lymphocytes, pubmed-meshheading:16237098-Dendritic Cells, pubmed-meshheading:16237098-Egg Proteins, pubmed-meshheading:16237098-Genetic Vectors, pubmed-meshheading:16237098-Humans, pubmed-meshheading:16237098-Interferon Type I, pubmed-meshheading:16237098-Mice, pubmed-meshheading:16237098-Mice, Inbred C3H, pubmed-meshheading:16237098-Mice, Inbred C57BL, pubmed-meshheading:16237098-Ovalbumin, pubmed-meshheading:16237098-Pan troglodytes, pubmed-meshheading:16237098-Peptide Fragments, pubmed-meshheading:16237098-Protein Biosynthesis, pubmed-meshheading:16237098-Receptor, Interferon alpha-beta, pubmed-meshheading:16237098-Receptors, Interferon, pubmed-meshheading:16237098-Signal Transduction, pubmed-meshheading:16237098-Toll-Like Receptor 4, pubmed-meshheading:16237098-Transcription, Genetic, pubmed-meshheading:16237098-Vaccination
pubmed:year
2005
pubmed:articleTitle
Dendritic cell maturation, but not CD8+ T cell induction, is dependent on type I IFN signaling during vaccination with adenovirus vectors.
pubmed:affiliation
Cell and Molecular Biology Group, Department of Medicine, University of Pennsylvania, Philadelphia, 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural