Source:http://linkedlifedata.com/resource/pubmed/id/16237076
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2005-10-20
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pubmed:abstractText |
A peptide encompassing residues 131-151 of the spliceosomal U1-70K protein and its analog phosphorylated at Ser140 were synthesized as potential candidates for the treatment of patients with lupus. Studies in the MRL/lpr and (NZB x NZW)F1 lupus models have demonstrated that these sequences contain a CD4+ T cell epitope but administration of the phosphorylated peptide only ameliorates the clinical manifestations of treated MRL/lpr mice. Binding assays with soluble HLA class II molecules and molecular modeling experiments indicate that both peptides behave as promiscuous epitopes and bind to a large panel of human DR molecules. In contrast to normal T cells and T cells from non-lupus autoimmune patients, we found that PBMCs from 40% of lupus patients selected randomly and CFSE-labeled CD4+ T cells proliferate in response to peptide 131-151. Remarkably, however, we observed that phosphorylation of Ser140 prevents CD4+ T cells proliferation but not secretion of regulatory cytokines, suggesting a striking immunomodulatory effect of phosphorylated analog on lupus CD4+ T cells that was unique to patients. The analog might act as an activator of regulatory T cells or as a partial agonist of TCR.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoprotein, U1 Small Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/SNRNP70 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5839-47
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pubmed:dateRevised |
2011-10-26
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pubmed:meshHeading |
pubmed-meshheading:16237076-Amino Acid Sequence,
pubmed-meshheading:16237076-CD4-Positive T-Lymphocytes,
pubmed-meshheading:16237076-HLA-DR Antigens,
pubmed-meshheading:16237076-Humans,
pubmed-meshheading:16237076-Interleukin-10,
pubmed-meshheading:16237076-Lupus Erythematosus, Systemic,
pubmed-meshheading:16237076-Lymphocyte Activation,
pubmed-meshheading:16237076-Molecular Sequence Data,
pubmed-meshheading:16237076-Peptide Fragments,
pubmed-meshheading:16237076-Phosphorylation,
pubmed-meshheading:16237076-Ribonucleoprotein, U1 Small Nuclear
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pubmed:year |
2005
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pubmed:articleTitle |
Selective modulation of CD4+ T cells from lupus patients by a promiscuous, protective peptide analog.
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pubmed:affiliation |
Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche 9021, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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