Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
44
pubmed:dateCreated
2005-11-2
pubmed:abstractText
An 11-residue peptide with the sequence DSLEFIASKLA was identified from a genomic library of Bacillus subtilis by phage display as an efficient substrate for Sfp phosphopantetheinyl transferase-catalyzed protein labeling by small molecule-CoA conjugates. We name this peptide the "ybbR tag," because part of its sequence is derived from the ybbR ORF in the B. subtilis genome. The site of Sfp-catalyzed ybbR tag labeling was mapped to the underlined Ser residue, and the ybbR tag was found to have a strong tendency for adopting an alpha-helical conformation in solution. Here we demonstrate that the ybbR tag can be fused to the N or C termini of target proteins or inserted in a flexible loop in the middle of a target protein for site-specific protein labeling by Sfp. The short size of the ybbR tag and its compatibility with various target proteins, the broad substrate specificity of Sfp for labeling the ybbR tag with small-molecule probes of diverse structures, and the high specificity and efficiency of the labeling reaction make Sfp-catalyzed ybbR tag labeling an attractive tool for expanding protein structural and functional diversities by posttranslational modification.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-10205056, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-10211839, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-10500161, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-10769129, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-10801328, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-11867633, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-12022841, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-12137509, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-12469133, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-12705828, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15065855, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15123281, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15212504, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15248752, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15264811, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15493886, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15653323, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15782206, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-15934048, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-16089439, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-16183024, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-16407987, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-3443622, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-3915782, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-5346390, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-8939706, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-8939709, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-9214294, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-9384377, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-9484229, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-9657724, http://linkedlifedata.com/resource/pubmed/commentcorrection/16236721-9759496
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
102
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15815-20
pubmed:dateRevised
2010-9-20
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase.
pubmed:affiliation
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural