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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-10-19
pubmed:abstractText
A mathematical model describing intracellular lysine synthesis by Corynebacterium glutamicum in batch fermentation was developed. The model is based on material balance equations of the key metabolites, and includes mechanistically based, experimentally matched rate equations for individual enzymes. From the measurements of the levels of intra- and extracellular metabolites during cultivation, the kinetic parameters in the model were identified through the decomposition of the network of reactions. The model predictions and experimental observations were in reasonable agreement. Using the model developed, metabolic control analysis was carried out to identify the rate-limiting steps, by evaluating the control on the overall lysine synthesis flux exerted by individual enzymatic reactions, which suggested how the control on lysine synthesis changes from aspartokinase to lysine permease as fermentation proceeded and indicated that lysine production could be enhanced by improving aspartokinase activity of this strain through genetic manipulation.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:issn
1389-1723
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-403
pubmed:year
1999
pubmed:articleTitle
Development of a kinetic model for L-lysine biosynthesis in Corynebacterium glutamicum and its application to metabolic control analysis.
pubmed:affiliation
Department of Biochemical Engineering & Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
pubmed:publicationType
Journal Article