Source:http://linkedlifedata.com/resource/pubmed/id/16231187
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2005-12-21
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pubmed:abstractText |
In forensic DNA analysis, improvement of DNA typing technologies has always been an issue. It has been shown that DNA amplification in low volumes is a suitable way to enhance the sensitivity and efficiency of amplification. In this study, DNA amplification was performed on a flat, chemically structured glass slide in 1-microl reaction volumes from cell line DNA contents between 1,000 and 4 pg. On-chip DNA amplification reproducibly yielded full allelic profiles from as little as 32 pg of template DNA. Applicability on the simultaneous amplification of 15 short tandem repeats and of a segment of the Amelogenin gene, which are routinely used in forensic DNA analysis, is shown. The results are compared to conventional in-tube amplification carried out in 25-microl reaction volumes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0937-9827
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
120
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
42-8
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pubmed:meshHeading |
pubmed-meshheading:16231187-Case-Control Studies,
pubmed-meshheading:16231187-DNA,
pubmed-meshheading:16231187-DNA Fingerprinting,
pubmed-meshheading:16231187-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:16231187-Feasibility Studies,
pubmed-meshheading:16231187-Female,
pubmed-meshheading:16231187-Humans,
pubmed-meshheading:16231187-Polymerase Chain Reaction
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pubmed:year |
2006
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pubmed:articleTitle |
Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit.
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pubmed:affiliation |
Institute of Legal Medicine, Albert Ludwig University Freiburg, Albertstrasse 9, 79104, Freiburg, Germany. ulrike.schmidt@uniklinik-freiburg.de
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pubmed:publicationType |
Journal Article
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