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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2006-1-18
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pubmed:databankReference |
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pubmed:abstractText |
As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix bundle that binds to one face of an A-form RNA duplex independently of sequence. These results suggest that B2 blocks both cleavage of the FHV genome by Dicer and incorporation of FHV small interfering RNAs into the RNA-induced silencing complex.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1545-9993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
952-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16228003-Crystallography,
pubmed-meshheading:16228003-Dimerization,
pubmed-meshheading:16228003-Models, Molecular,
pubmed-meshheading:16228003-Nodaviridae,
pubmed-meshheading:16228003-Protein Binding,
pubmed-meshheading:16228003-Protein Conformation,
pubmed-meshheading:16228003-RNA, Double-Stranded,
pubmed-meshheading:16228003-RNA, Small Interfering,
pubmed-meshheading:16228003-RNA Interference,
pubmed-meshheading:16228003-RNA-Binding Proteins,
pubmed-meshheading:16228003-Ribonuclease III,
pubmed-meshheading:16228003-Structure-Activity Relationship,
pubmed-meshheading:16228003-Viral Proteins
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pubmed:year |
2005
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pubmed:articleTitle |
Dual modes of RNA-silencing suppression by Flock House virus protein B2.
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pubmed:affiliation |
Department of Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, California 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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