Source:http://linkedlifedata.com/resource/pubmed/id/16227676
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2005-10-17
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| pubmed:abstractText |
The R-3000 reticulocyte analyzer uses flow cytometry with an argon laser as its light source. This analyzer stains residual RNA with auramine O to provide a reticulocyte maturation differential. Using the R-3000, we analyzed 119 samples of bone marrow (BM) and peripheral blood (PB) from 111 patients with hematologic disorders. Parameters were reticulocytes, immature reticulocyte fraction (IRF) percentage in BM and PB, BM/PB reticulocyte ratio, and BM/PB IRF ratio. Reticulocytes and IRF percentage in BM were significantly higher than in PB (p < 0.01). There was also a good correlation between reticulocyte percentages in BM and in PB (r = 0.81). Patients were classified into a normal group (without anemia) and an anemia group. Furthermore, the anemia group was classified into three groups: group 1: cases with hematopoietic dysfunction; group 2: cases in bone marrow recovery phase after chemotherapy and hematopoietic stem cell transplantation, and hematologic disorders with bone marrow accelerative phase, and group 3: cases with ineffective hematopoiesis (myelodysplastic syndrome). The mean reticulocyte percentage of the normal group was 2.3 +/- 1.1%, which was close to the normal value in BM. The BM/PB reticulocyte ratio of group 3 was statistically higher than that of groups 1 and 2. This indicates that group 3 had ineffective erythropoiesis and that the BM/PB ratio is a useful indicator for the diagnosis of myelodysplastic syndrome.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0001-5792
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| pubmed:author | |
| pubmed:copyrightInfo |
(c) 2005 S. Karger AG, Basel
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| pubmed:issnType |
Print
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| pubmed:volume |
114
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
141-5
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| pubmed:meshHeading |
pubmed-meshheading:16227676-Bone Marrow Cells,
pubmed-meshheading:16227676-Cell Differentiation,
pubmed-meshheading:16227676-Female,
pubmed-meshheading:16227676-Hematologic Diseases,
pubmed-meshheading:16227676-Humans,
pubmed-meshheading:16227676-Male,
pubmed-meshheading:16227676-Middle Aged,
pubmed-meshheading:16227676-Reticulocyte Count,
pubmed-meshheading:16227676-Reticulocytes
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| pubmed:year |
2005
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| pubmed:articleTitle |
Automated analyzer evaluation of reticulocytes in bone marrow and peripheral blood of hematologic disorders.
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| pubmed:affiliation |
Clinical Hematology and Clinical Diagnostics, Osaka City University, Graduate School of Medicine, Osaka, Japan.
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| pubmed:publicationType |
Journal Article
|