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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
2005-10-17
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pubmed:abstractText |
Vertebrate genomes each encode hundreds of micro-RNAs (miRNAs), yet for few of these miRNAs is there empirical evidence as to which mRNA(s) they regulate. Here we report the identification of human lin-28 mRNA as a regulatory target of human miR-125b and its homolog miR-125a. Studies of miR-125b function in mouse P19 embryonal carcinoma cells induced to develop into neurons suggest a role for this regulatory miRNA in mammalian neuronal differentiation, since its increased concentration in these cells contributes to lin-28 downregulation. Within the lin-28 3' untranslated region (UTR) are two conserved miRNA responsive elements (miREs) that mediate repression by miR-125b and miR-125a. Simultaneous deletion of both miREs renders the lin-28 3' UTR almost completely insensitive to these miRNAs, indicating that these two miREs are the principal elements in the lin-28 3' UTR that respond to miR-125. At the 3' end of each element is an adenosine residue that makes a significant contribution to function irrespective of its complementarity to the 5'-terminal nucleotide of miR-125. By contrast to most earlier reports of gene repression by other miRNAs that are imperfectly complementary to their targets, lin-28 downregulation by miR-125 involves reductions in both translational efficiency and mRNA abundance. The decrease in the mRNA concentration is achieved by a posttranscriptional mechanism that is independent of the inhibitory effect on translation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-10617568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-10642801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-10706289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-10778853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11081512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11157775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11452083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11461699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11498593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11641272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11679670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11884032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11914277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-11959843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-12007417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-12086629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-12101121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-12110901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-12600936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-12624257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-12737800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-12798299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-13130141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-14508493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-14643679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-14697198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-14970384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-14970398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15003116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15014042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15024405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15105502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15131085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15297624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15311210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15453917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15502875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15538371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15585662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15652477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15652478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15685193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15722555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15723116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15766527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15944709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-15951802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-2496006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-6494891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-7107698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-8252621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-8252622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-8377226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-8957004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-9054503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16227573-9860998
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9198-208
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16227573-Humans,
pubmed-meshheading:16227573-Animals,
pubmed-meshheading:16227573-Mice,
pubmed-meshheading:16227573-Neurons,
pubmed-meshheading:16227573-Cell Differentiation,
pubmed-meshheading:16227573-RNA, Messenger,
pubmed-meshheading:16227573-Cell Line,
pubmed-meshheading:16227573-Neoplastic Stem Cells,
pubmed-meshheading:16227573-Down-Regulation,
pubmed-meshheading:16227573-RNA-Binding Proteins,
pubmed-meshheading:16227573-3' Untranslated Regions,
pubmed-meshheading:16227573-Embryonal Carcinoma Stem Cells,
pubmed-meshheading:16227573-Genes, Reporter
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