Source:http://linkedlifedata.com/resource/pubmed/id/16226120
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2005-10-17
|
| pubmed:abstractText |
Human trophoblast research relies on a combination of in vitro models, including isolated primary cultures, explant cultures, and trophoblast cell lines. In the present study, we have utilized the rotating wall vessel (RWV) bioreactor to generate a three-dimensional (3-D) model of human placentation for the study of cytotrophoblast (CTB) invasion. The RWV supported the growth of the human CTB cell line SGHPL-4 and allowed for the formation of complex, multilayered 3-D aggregates that were morphologically, phenotypically, and functionally distinct from SGHPL-4 monolayers. The cells cultured three-dimensionally differentiated into an aggressively invasive cell population characterized by the upregulation of matrix metalloproteinase-2 (MMP-2), MMP-3, MMP-9 and urokinase-type plasminogen activator (uPA) secretion and activation. Microarray analysis of the 3-D and 2-D cultured cells revealed increased expression in the 3-D cells of various genes that are known mediators of invasion, including MT1-MMP, PECAM-1 and L-selectin, as well as genes not previously associated with CTB differentiation such as MMP-13 and MT5-MMP. These results were verified by quantitative real-time PCR. These findings suggest that when cultured in 3-D, SGHPL-4 cells closely mimic differentiating in utero CTBs, providing a novel approach for the in vitro study of the molecular mechanisms that regulate CTB differentiation and invasion.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD31,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Urokinase-Type Plasminogen Activator
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
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| pubmed:issn |
0143-4004
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
709-20
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:16226120-Antigens, CD31,
pubmed-meshheading:16226120-Bioreactors,
pubmed-meshheading:16226120-Blotting, Western,
pubmed-meshheading:16226120-Cell Aggregation,
pubmed-meshheading:16226120-Cell Differentiation,
pubmed-meshheading:16226120-Cell Growth Processes,
pubmed-meshheading:16226120-Cell Line,
pubmed-meshheading:16226120-Female,
pubmed-meshheading:16226120-Humans,
pubmed-meshheading:16226120-L-Selectin,
pubmed-meshheading:16226120-Matrix Metalloproteinases,
pubmed-meshheading:16226120-Microscopy, Electron, Scanning,
pubmed-meshheading:16226120-Microscopy, Fluorescence,
pubmed-meshheading:16226120-Placentation,
pubmed-meshheading:16226120-Pregnancy,
pubmed-meshheading:16226120-RNA, Messenger,
pubmed-meshheading:16226120-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16226120-Trophoblasts,
pubmed-meshheading:16226120-Urokinase-Type Plasminogen Activator
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Three-dimensional growth of extravillous cytotrophoblasts promotes differentiation and invasion.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, Tulane University Health Sciences Center, New Orleans, LA, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|