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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
43
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pubmed:dateCreated |
2005-10-26
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pubmed:abstractText |
The costimulatory ligands B7-1 and B7-2 are expressed on the surface of antigen-presenting cells and interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells. Although B7-1 and B7-2 are homologous ligands having common receptors, they exhibit distinct biochemical features and roles in immune regulation. Several biochemical and structural studies have indicated differences in the oligomeric state of B7-1 and B7-2. However, the organization of B7 ligands on the cell surface has not been examined. By using photobleaching-based FRET (pbFRET), we demonstrate that B7-1 and B7-2 adopt different oligomeric states on the cell surface. Our study shows that B7-2 exists as a monomer on the cell surface whereas B7-1 exists predominantly as dimers on the cell surface. A series of mutations in B7-1 result in the expression of a predominantly monomeric species on the cell surface and validate the dimer interface proposed by prior crystallographic analysis. The difference in the oligomeric states of B7-1 and B7-2 provides insight into the geometric organization of the costimulatory receptor-ligand complexes in the immunological synapse and suggests constraints on signal transduction mechanisms involved in T cell activation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-10399144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-10661405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-10875917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-10875918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-11163182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-11279501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-11279502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-11359816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-11403577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-11713465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-11726649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-11825563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-12368911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-12606712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-12615890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-14595367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-15030777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-15034022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-15357951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-15467723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-15628877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-15696168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-15771580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-1847722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-2465550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-2660827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-2794510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-7481803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-7534215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-7541042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-7584144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-7694363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-7882171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-7964482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-8180176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-8717514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-8793565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-8900156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-9188449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-9660864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16221763-9725457
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15569-74
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16221763-Animals,
pubmed-meshheading:16221763-Antigens, CD,
pubmed-meshheading:16221763-Antigens, CD28,
pubmed-meshheading:16221763-Antigens, CD80,
pubmed-meshheading:16221763-Antigens, CD86,
pubmed-meshheading:16221763-Antigens, Differentiation,
pubmed-meshheading:16221763-CHO Cells,
pubmed-meshheading:16221763-CTLA-4 Antigen,
pubmed-meshheading:16221763-Cricetinae,
pubmed-meshheading:16221763-Dimerization,
pubmed-meshheading:16221763-Fluorescence Resonance Energy Transfer,
pubmed-meshheading:16221763-Lymphocyte Activation,
pubmed-meshheading:16221763-Signal Transduction,
pubmed-meshheading:16221763-T-Lymphocytes
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pubmed:year |
2005
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pubmed:articleTitle |
Different cell surface oligomeric states of B7-1 and B7-2: implications for signaling.
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pubmed:affiliation |
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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