Source:http://linkedlifedata.com/resource/pubmed/id/16219982
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
2005-10-12
|
| pubmed:abstractText |
Insulin-like growth factor-I (IGF-I) gene generates several IGF-I mRNA variants by alternative splicing. Two promoters are present in mouse IGF-I gene. Each promoter encodes two IGF-I mRNA variants (IGF-IA and IGF-IB mRNAs). Variants differ by the presence (IGF-IB) or absence (IGF-IA) of a 52-bp insert in the E domain-coding region. Functional differences among IGF-I mRNAs, and regulatory mechanisms for alternative splicing of IGF-I mRNA are not yet known. We analyzed the expression of mouse IGF-IA and IGF-IB mRNAs using SYBR Green real-time RT-PCR. In the liver, IGF-I mRNA expression increased from 10 days of age to 45 days. In the uterus and ovary, IGF-I mRNA expression increased from 21 days of age, and then decreased at 45 days. In the kidney, IGF-I mRNA expression decreased from 10 days of age. IGF-IA mRNA levels were higher than IGF-IB mRNA levels in all organs examined. Estradiol-17beta (E2) treatment in ovariectomized mice increased uterine IGF-IA and IGF-IB mRNA levels from 3 hr after injection, and highest levels for both mRNAs were detected at 6 hr, and relative increase was greater for IGF-IB mRNA than for IGF-IA mRNA. These results suggest that expression of IGF-I mRNA variants is regulated in organ-specific and age-dependent manners, and estrogen is involved in the change of IGF-I mRNA variant expression.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0289-0003
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1011-21
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:16219982-Age Factors,
pubmed-meshheading:16219982-Alternative Splicing,
pubmed-meshheading:16219982-Animals,
pubmed-meshheading:16219982-DNA Primers,
pubmed-meshheading:16219982-Estradiol,
pubmed-meshheading:16219982-Female,
pubmed-meshheading:16219982-Gene Expression,
pubmed-meshheading:16219982-Kidney,
pubmed-meshheading:16219982-Liver,
pubmed-meshheading:16219982-Male,
pubmed-meshheading:16219982-Mice,
pubmed-meshheading:16219982-Organ Specificity,
pubmed-meshheading:16219982-Ovary,
pubmed-meshheading:16219982-Promoter Regions, Genetic,
pubmed-meshheading:16219982-RNA, Messenger,
pubmed-meshheading:16219982-Receptor, IGF Type 1,
pubmed-meshheading:16219982-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16219982-Uterus
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Organ-specific and age-dependent expression of insulin-like growth factor-I (IGF-I) mRNA variants: IGF-IA and IB mRNAs in the mouse.
|
| pubmed:affiliation |
Department of Biology, Faculty of Science, Okayama University, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|