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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-8-6
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pubmed:databankReference |
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pubmed:abstractText |
We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 M guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
202
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-95
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1621990-Aggrecans,
pubmed-meshheading:1621990-Amino Acid Sequence,
pubmed-meshheading:1621990-Animals,
pubmed-meshheading:1621990-Autoradiography,
pubmed-meshheading:1621990-Base Sequence,
pubmed-meshheading:1621990-Blotting, Northern,
pubmed-meshheading:1621990-Cartilage, Articular,
pubmed-meshheading:1621990-Chromatography, Affinity,
pubmed-meshheading:1621990-Collagen,
pubmed-meshheading:1621990-DNA Probes,
pubmed-meshheading:1621990-Dogs,
pubmed-meshheading:1621990-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1621990-Extracellular Matrix Proteins,
pubmed-meshheading:1621990-Lectins, C-Type,
pubmed-meshheading:1621990-Molecular Sequence Data,
pubmed-meshheading:1621990-Nucleic Acid Hybridization,
pubmed-meshheading:1621990-Polymerase Chain Reaction,
pubmed-meshheading:1621990-Procollagen,
pubmed-meshheading:1621990-Protein Biosynthesis,
pubmed-meshheading:1621990-Proteoglycans,
pubmed-meshheading:1621990-RNA, Messenger,
pubmed-meshheading:1621990-Ultracentrifugation
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pubmed:year |
1992
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pubmed:articleTitle |
Extraction and isolation of mRNA from adult articular cartilage.
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pubmed:affiliation |
Department of Medicine, University of Calgary, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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