Linked Life Data

16219633

Source:http://linkedlifedata.com/resource/pubmed/id/16219633

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-2-27
pubmed:abstractText
Oral squamous cell carcinoma is one of the most common human neoplasms, and prevention of this malignancy requires a better understanding of its carcinogenesis process. To this end, we tried to establish an animal model using the human c-Ha-ras proto-oncogene-carrying transgenic (Tg) rats and the carcinogen 4-nitroquinoline 1-oxide (4-NQO). 4-NQO (20 p.p.m.) was administered to Tg and non-Tg rats for 8 weeks in their drinking water, and then the occurrence of tongue carcinogenesis was compared during the experimental period of 22 weeks. In addition, we determined the DNA ploidy in tongue lesions and examined the immunohistochemical expression of five biomarkers such as cyclin D1, glutathione S-transferase placental form, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) and beta-catenin. Next, the cancer chemopreventive effects of nimesulide, pioglitazone and a synthetic geranylated derivative, which have been reported to be inhibitors of tongue carcinogenesis, were examined in Tg rats treated with 4-NQO. Either during or after treatment with 4-NQO in the drinking water, tongue dysplasia and tumors were observed on the tongues of both Tg and non-Tg rats, with a greater incidence and multiplicity in Tg rats. Histopathologically, squamous cell dysplasia, papilloma and carcinoma with or without invasion were present in the tongue. Immunohistochemistry revealed that expression levels against five biomarkers increase with disease progression, and the changes correlated with those of the DNA ploidy pattern. Interestingly, a strong expression of COX-2, iNOS and beta-catenin was observed on the invasive front of squamous cell carcinomas. A subsequent chemoprevention study using Tg rats showed that the chemicals tested suppressed the occurrence of tongue carcinomas when they were administered after 4-NQO-exposure. These results may thus indicate that our 4-NQO-induced Tg rat tongue carcinogenesis model simulates many aspects of human oral carcinogenesis and it can be applied for an analysis of oral cancer development while also helping to identify potentially effective cancer chemopreventive agents against oral cancer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
619-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16219633-4-Nitroquinoline-1-oxide, pubmed-meshheading:16219633-Administration, Oral, pubmed-meshheading:16219633-Animals, pubmed-meshheading:16219633-Animals, Genetically Modified, pubmed-meshheading:16219633-Carcinogens, pubmed-meshheading:16219633-Cell Transformation, Neoplastic, pubmed-meshheading:16219633-Chemoprevention, pubmed-meshheading:16219633-Cyclooxygenase 2, pubmed-meshheading:16219633-Disease Models, Animal, pubmed-meshheading:16219633-Disease Progression, pubmed-meshheading:16219633-Gene Expression Profiling, pubmed-meshheading:16219633-Humans, pubmed-meshheading:16219633-Immunohistochemistry, pubmed-meshheading:16219633-Nitric Oxide Synthase Type II, pubmed-meshheading:16219633-Ploidies, pubmed-meshheading:16219633-Rats, pubmed-meshheading:16219633-Tongue Neoplasms, pubmed-meshheading:16219633-beta Catenin
pubmed:year
2006
pubmed:articleTitle
An animal model for the rapid induction of tongue neoplasms in human c-Ha-ras proto-oncogene transgenic rats by 4-nitroquinoline 1-oxide: its potential use for preclinical chemoprevention studies.
pubmed:affiliation
Department of Oncologic Pathology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 920-0293, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't