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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2005-10-10
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pubmed:abstractText |
Fluorescence quenching by guanine allows DNA hybridization to be monitored and any point mutations in oligonucleotides to be detected. However, fluorescence quenching is often affected by untargeted guanine located in a protruding end (single-strand DNA) of the probe-target DNA duplex resulting in an unsatisfactory sensitivity. In the present study, we used enzymatic digestion of the protruding end of a probe-target DNA duplex to avoid interference by untargeted guanine on fluorescence quenching for detection of a nucleobase mutation. Enzymatic digestion of the protruding end of the DNA duplex fully prevented interference by untargeted guanine, and produced a marked difference in the quenching ratios (36% for wild-type, and 0% for mutant).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-carboxyfluorescein,
http://linkedlifedata.com/resource/pubmed/chemical/5-carboxytetramethylrhodamine...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Exodeoxyribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Rhodamines,
http://linkedlifedata.com/resource/pubmed/chemical/gene b exonuclease
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0141-5492
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1349-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16215848-Base Sequence,
pubmed-meshheading:16215848-DNA, Single-Stranded,
pubmed-meshheading:16215848-DNA-Directed DNA Polymerase,
pubmed-meshheading:16215848-Exodeoxyribonucleases,
pubmed-meshheading:16215848-Fluorescein-5-isothiocyanate,
pubmed-meshheading:16215848-Fluoresceins,
pubmed-meshheading:16215848-Fluorescence,
pubmed-meshheading:16215848-Fluorescence Resonance Energy Transfer,
pubmed-meshheading:16215848-Guanosine Triphosphate,
pubmed-meshheading:16215848-Hepatitis B virus,
pubmed-meshheading:16215848-Humans,
pubmed-meshheading:16215848-Molecular Sequence Data,
pubmed-meshheading:16215848-Nucleic Acid Hybridization,
pubmed-meshheading:16215848-Oligodeoxyribonucleotides,
pubmed-meshheading:16215848-Oligonucleotide Probes,
pubmed-meshheading:16215848-Point Mutation,
pubmed-meshheading:16215848-Rhodamines
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pubmed:year |
2005
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pubmed:articleTitle |
Mutation detection in DNA oligonucleotides based on a Guanine quenching method coupled with enzymatic digestion of single-stranded DNA.
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pubmed:affiliation |
Department of Applied Chemistry Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki, 812-8581, Fukuoka, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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