Linked Life Data

16214731

Source:http://linkedlifedata.com/resource/pubmed/id/16214731

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-10-10
pubmed:abstractText
The programmed cell death (PCD, the apoptosis) is associated with the activation of the "death enzymes" caspases and very likely plays a role in the decrease of sperm vitality during cryopreservation. The activation of pan-caspases was examined in fresh and cryostored sperm by fluorescence microscopy applying a cell permeable and noncytotoxic carboxy-fluorescein labelled caspase inhibitor. Cryopreservation increased significantly the percentage of spermatozoa with activated pan-caspases (aCP) from 21 +/- 2.6 to 47 +/- 5.8 % (mean +/- SEM). Quantitative Western blot analyses confirmed the activation of caspase-1, -8, and -9 in detail on protein level after cryopreservation, whereas, the caspases of sperm of infertility patients showed a higher activation status than donors' sperm. This effect raised with increasing glycerol concentration from 7-14 %. The higher level in activation of caspases in cryostored spermatozoa of infertility patients may indicate that these cells have a lower cryotolerance and a higher susceptibility to caspase activation than does the sperm of donors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0148-5016
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
449-60
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Sperm caspases become more activated in infertility patients than in healthy donors during cryopreservation.
pubmed:affiliation
Division of Andrology, Department of Dermatology, University of Leipzig, Leipzig, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't