Linked Life Data

16213987

Source:http://linkedlifedata.com/resource/pubmed/id/16213987

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-10-10
pubmed:abstractText
To determine whether neural precursor cells have region-specific growth properties, we compared the proliferation, mitogenicity, and differentiation of these cells isolated from the embryonic day 16 rat forebrain and spinal cord. Neural precursor cells isolated from both regions were cultured in growth medium supplemented with epidermal growth factor, basic fibroblast growth factor, or epidermal growth factor+basic fibroblast growth factor. Under all three conditions, both neural precursor cell populations proliferated for multiple passages. While spinal cord-derived neural precursor cells proliferated moderately faster in epidermal growth factor-enriched growth medium, brain-derived cells proliferated much faster in basic fibroblast growth factor-enriched growth medium. When exposed to both epidermal growth factor and basic fibroblast growth factor, the two neural precursor cell populations expanded and proliferated more rapidly than when exposed to a single factor, with brain-derived neural precursor cells expanding significantly faster than spinal cord-derived ones (P<0.0001). Differentiation studies showed that both neural precursor cell populations were multi-potent giving rise to neurons, astrocytes, and oligodendrocytes. However, neuronal differentiation from brain-derived neural precursor cells was greater than spinal cord-derived ones (11.95+/-5.00% vs 1.92+/-1.13%; passage 2). Further, the two neural precursor cell populations differentiated into a similar percentage of oligodendrocytes (brain: 8.66+/-5.85%; spinal cord: 7.69+/-3.91%; passage 2). Immunofluorescence and Western blot studies showed that neural precursor cells derived from both regions expressed receptors for basic fibroblast growth factor and epidermal growth factor. However, brain-derived neural precursor cells expressed higher levels of the two receptors than spinal cord-derived ones in growth medium containing epidermal growth factor+basic fibroblast growth factor. Thus, our results showed that neural precursor cells isolated from the two regions of the CNS have distinct properties and growth requirements. Identifying phenotypic differences between these neural precursor cell populations and their growth requirements should provide new insights into the development of cell therapies for region-specific neurological degenerative diseases.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
851-62
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16213987-Animals, pubmed-meshheading:16213987-Blotting, Western, pubmed-meshheading:16213987-Brain, pubmed-meshheading:16213987-Cell Differentiation, pubmed-meshheading:16213987-Cell Proliferation, pubmed-meshheading:16213987-Cell Separation, pubmed-meshheading:16213987-Culture Media, pubmed-meshheading:16213987-Fluorescent Antibody Technique, pubmed-meshheading:16213987-Glial Fibrillary Acidic Protein, pubmed-meshheading:16213987-Neurons, pubmed-meshheading:16213987-Oligodendroglia, pubmed-meshheading:16213987-Prosencephalon, pubmed-meshheading:16213987-Protein-Serine-Threonine Kinases, pubmed-meshheading:16213987-Rats, pubmed-meshheading:16213987-Rats, Wistar, pubmed-meshheading:16213987-Spinal Cord, pubmed-meshheading:16213987-Stem Cells, pubmed-meshheading:16213987-Tubulin, pubmed-meshheading:16213987-Tumor Necrosis Factor Receptor-Associated Peptides and...
pubmed:year
2005
pubmed:articleTitle
Region-specific growth properties and trophic requirements of brain- and spinal cord-derived rat embryonic neural precursor cells.
pubmed:affiliation
Department of Neurobiology, Shanghai Second Medical University, 280 South Chong-Qing Road, Shanghai 200025, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural