Linked Life Data

16212947

Source:http://linkedlifedata.com/resource/pubmed/id/16212947

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-10-21
pubmed:abstractText
The two structurally related nuclear receptor corepressor (N-CoR) and silencing mediator of retinoid and thyroid receptors (SMRT) proteins have been found to differentially affect the transcriptional activity of numerous nuclear receptors, such as thyroid hormone, retinoic acid and steroid receptors. Because of the numerous effects mediated by nuclear receptors in brain, it is of interest to extend these in vitro data and to explore the cellular distribution of both corepressors in brain tissue. We therefore examined, using in situ hybridisation, whether the relative abundance of these two functionally distinct corepressors differed in rat brain and pituitary. We find that although both N-CoR and SMRT transcripts are ubiquitously expressed in brain, striking differences in their respective levels of expression could be observed in discrete areas of brain stem, thalamus, hypothalamus and hippocampus. Using dual-label immunofluorescence, we examined in selected glucocorticoid sensitive areas involved in the regulation of the hypothalamus-pituitary-adrenal axis activity, the respective protein abundance of N-CoR and SMRT. Protein abundance was largely concurrent with the mRNA expression levels, with SMRT relatively more abundant in hypothalamus and brain stem areas. Colocalisation of N-CoR and SMRT was demonstrated by confocal microscopy in most areas studied. Taken together, these findings are consistent with the idea that the uneven neuroanatomical distribution of N-CoR and SMRT protein may contribute to the site-specific effects exerted by hormones, such as glucocorticoids, in the brain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
1059
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-21
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16212947-Animals, pubmed-meshheading:16212947-Brain, pubmed-meshheading:16212947-Brain Mapping, pubmed-meshheading:16212947-DNA-Binding Proteins, pubmed-meshheading:16212947-Hypothalamo-Hypophyseal System, pubmed-meshheading:16212947-In Situ Hybridization, pubmed-meshheading:16212947-Male, pubmed-meshheading:16212947-Nuclear Proteins, pubmed-meshheading:16212947-Nuclear Receptor Co-Repressor 1, pubmed-meshheading:16212947-Nuclear Receptor Co-Repressor 2, pubmed-meshheading:16212947-Pituitary Gland, pubmed-meshheading:16212947-Pituitary-Adrenal System, pubmed-meshheading:16212947-RNA, Messenger, pubmed-meshheading:16212947-Rats, pubmed-meshheading:16212947-Rats, Wistar, pubmed-meshheading:16212947-Repressor Proteins, pubmed-meshheading:16212947-Signal Transduction, pubmed-meshheading:16212947-Tissue Distribution, pubmed-meshheading:16212947-Transcription, Genetic
pubmed:year
2005
pubmed:articleTitle
Neuroanatomical distribution and colocalisation of nuclear receptor corepressor (N-CoR) and silencing mediator of retinoid and thyroid receptors (SMRT) in rat brain.
pubmed:affiliation
Division of Medical Pharmacology, Leiden/Amsterdam Centre for Drug Research and Leiden University Medical Center, The Netherlands. s.van.der.laan@lacdr.leidenuniv.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't