Linked Life Data

16211655

Source:http://linkedlifedata.com/resource/pubmed/id/16211655

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2005-10-19
pubmed:abstractText
Salivary cortisol concentration seems to be an excellent indicator of the biologically active plasma cortisol level because of the nearly absence of corticoid-binding proteins in saliva. With regard to the easy, noninvasive, and stress-free nature of saliva sampling, this parameter greatly facilitates studies of the HPA (hypothalamus-pituitary-adrenocortical) axis, especially in children. A commercially available TR-FIA (time-resolved fluoroimmunoassay), the DELFIA (dissociation-enhanced lanthanide fluoroimmunoassay) method, proposed for plasma and urine cortisol analyses, was adapted for salivary cortisol measurement by means of simple modifications of the assay protocol. The sensitivity was determined to be 0.53 nmol/L. The intra- and interassay coefficients of variation ranged from 5.5 to 8.2 % and from 6.0 to 10.4 %, respectively. The present findings suggest that the DELFIA procedure provides a reliable, sensitive, and convenient alternative procedure to the assay for salivary cortisol.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0365-6233
pubmed:author
pubmed:issnType
Print
pubmed:volume
338
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
493-7
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Adaptation of DELFIA cortisol kit for determination of salivary cortisol concentration.
pubmed:affiliation
Department of Clinical Pharmacy and Diagnostics, University of Vienna, Austria.
pubmed:publicationType
Journal Article