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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-1-17
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pubmed:abstractText |
Osteopontin is a multifunctional matricellular protein identified as one of the most upregulated genes in pulmonary fibrosis. Experimental animal models have identified early pro-fibrotic cytokines as essential to the pathogenesis of inflammation-induced pulmonary fibrosis. However, the principal sources of osteopontin in the fibroproliferative lung, and the factors responsible for its induction, have not been fully defined. We isolated primary rat lung fibroblasts in culture to examine the expression and regulation of lung fibroblast-derived osteopontin. Our results demonstrate a potent and dramatic increase in osteopontin expression induced by interleukin-1beta (IL-1beta), whereas tumor necrosis factor-alpha, transforming growth factor-beta, and angiotensin II had minimal effect. Stimulation with IL-1beta resulted in the secretion of soluble osteopontin protein. We found that osteopontin expression by IL-1beta was regulated via signaling primarily through the mitogen-activated protein kinase member ERK1/2, partially by p38 MAPK, but not at all by JNK. Finally, the mechanism of IL-1beta increase in osteopontin mRNA requires de novo transcription and translation. In conclusion, we find that osteopontin is expressed by primary lung fibroblasts and is potently upregulated by the early inflammatory and pro-fibrotic cytokine IL-1beta. Activated fibroblasts may be a significant source of osteopontin production during lung fibrogenesis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Osteopontin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Spp1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0730-2312
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pubmed:author |
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pubmed:copyrightInfo |
(c) 2005 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
519-29
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16211580-Angiotensin II,
pubmed-meshheading:16211580-Animals,
pubmed-meshheading:16211580-Animals, Newborn,
pubmed-meshheading:16211580-Cells, Cultured,
pubmed-meshheading:16211580-Fibroblasts,
pubmed-meshheading:16211580-Interleukin-1,
pubmed-meshheading:16211580-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:16211580-Lung,
pubmed-meshheading:16211580-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:16211580-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:16211580-Osteopontin,
pubmed-meshheading:16211580-RNA, Messenger,
pubmed-meshheading:16211580-Rats,
pubmed-meshheading:16211580-Rats, Sprague-Dawley,
pubmed-meshheading:16211580-Sialoglycoproteins,
pubmed-meshheading:16211580-Signal Transduction,
pubmed-meshheading:16211580-Transforming Growth Factor beta,
pubmed-meshheading:16211580-Tumor Necrosis Factor-alpha,
pubmed-meshheading:16211580-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2006
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pubmed:articleTitle |
Interleukin-1beta induces osteopontin expression in pulmonary fibroblasts.
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pubmed:affiliation |
Department of Medicine, Pulmonary Center, Boston University School of Medicine, 715 Albany Street R304, Boston, MA 02118, USA. daserlin@bu.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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