Source:http://linkedlifedata.com/resource/pubmed/id/16207746
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
421
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pubmed:dateCreated |
2005-10-18
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pubmed:abstractText |
A glycine-rich RNA-binding protein4 (GR-RBP4), one of the eight GR-RBP family members in Arabidopsis thaliana, was investigated for its stress-related expression, nucleic acid-binding property, and functional roles in plants subjected to various stresses including cold, high salinity, and dehydration. Real-time RT-PCR and GUS expression analyses showed that GR-RBP4 was abundantly expressed in young plants, root tips, and flowers, but weakly in mature leaves and stems, implying that GR-RBP4 is highly expressed in actively proliferating organs. The transcript level of GR-RBP4 increased markedly with cold stress, decreased significantly with salt stress, and decreased slightly with dehydration stress. In vitro nucleic acid-binding assays revealed that GR-RBP4 protein binds sequence non-specifically to RNAs and DNAs. Characterization of the transgenic Arabidopsis plants overexpressing GR-RBP4 under the control of the 35S promoter revealed that 35S::GR-RBP4 lines displayed retarded germination compared with the wild type under salt or dehydration stress. Despite the marked up-regulation of GR-RBP4 expression by cold stress, the 35S::GR-RBP4 lines did not show any noticeable changes in cold or freezing tolerance compared with wild-type plants. These results indicate that GR-RBP4 contributes differently to altered germination and seedling growth of Arabidopsis plants under various stress conditions.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Water
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-0957
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3007-16
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16207746-Arabidopsis,
pubmed-meshheading:16207746-Arabidopsis Proteins,
pubmed-meshheading:16207746-Cold Temperature,
pubmed-meshheading:16207746-Desiccation,
pubmed-meshheading:16207746-Gene Expression Regulation, Plant,
pubmed-meshheading:16207746-Multigene Family,
pubmed-meshheading:16207746-Nucleic Acids,
pubmed-meshheading:16207746-Plants, Genetically Modified,
pubmed-meshheading:16207746-RNA-Binding Proteins,
pubmed-meshheading:16207746-Sodium Chloride,
pubmed-meshheading:16207746-Water
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pubmed:year |
2005
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pubmed:articleTitle |
Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress.
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pubmed:affiliation |
Division of Applied Plant Science and Agricultural Plant Stress Research Center, College of Agriculture and Life Sciences, Chonnam National University, 300 Youngbong-Dong, Buk-gu, Gwangju 500-757, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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