Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-12-6
pubmed:abstractText
One group of proteins that regulates neurite outgrowth and maintains neuronal networks is the immunoglobulin superfamily (IgSF). We previously identified a new member of the IgSF, keratinocyte-associated transmembrane protein-4 (KCT-4), by the signal sequence-trap method from primary cultured human keratinocytes. The KCT-4 mRNA has been found to be highly expressed in the adult human brain, although it is also distributed in various tissues. In the present study, to gain insight into the role of KCT-4 in the nervous system, we examined the expression profile and localization of KCT-4 mRNA in mouse brain. We also evaluated changes in KCT-4 mRNA expression in the differentiation of the neuroblastoma cell line Neuro-2a as the in vitro model of neurite outgrowth. KCT-4 mRNA was detected broadly in various regions of the adult mouse brain by RT-PCR. In situ hybridization revealed that it was expressed highly selectively by neurons but not by glial cells. Moreover, expression of KCT-4 mRNA was induced by neurite outgrowth of Neuro-2a. These data suggest that KCT-4 participates in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
392
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
226-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16203089-Animals, pubmed-meshheading:16203089-Antigens, CD, pubmed-meshheading:16203089-Blotting, Northern, pubmed-meshheading:16203089-Brain, pubmed-meshheading:16203089-Cell Line, Tumor, pubmed-meshheading:16203089-Gene Expression, pubmed-meshheading:16203089-Glial Fibrillary Acidic Protein, pubmed-meshheading:16203089-Immunoglobulins, pubmed-meshheading:16203089-Immunohistochemistry, pubmed-meshheading:16203089-In Situ Hybridization, pubmed-meshheading:16203089-Membrane Proteins, pubmed-meshheading:16203089-Mice, pubmed-meshheading:16203089-Neurites, pubmed-meshheading:16203089-Neuroblastoma, pubmed-meshheading:16203089-Neurons, pubmed-meshheading:16203089-Phosphopyruvate Hydratase, pubmed-meshheading:16203089-RNA, Messenger, pubmed-meshheading:16203089-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16203089-S100 Proteins, pubmed-meshheading:16203089-Time Factors, pubmed-meshheading:16203089-Tretinoin, pubmed-meshheading:16203089-Up-Regulation
pubmed:year
2006
pubmed:articleTitle
Neuronal expression of keratinocyte-associated transmembrane protein-4, KCT-4, in mouse brain and its up-regulation by neurite outgrowth of Neuro-2a cells.
pubmed:affiliation
Department of Veterinary Clinical Pathology, Nippon Veterinary and Animal Science University, Musashino-shi, Tokyo 180-8602, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't