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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2005-10-5
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pubmed:abstractText |
The molecular mechanisms specifying the dendritic morphology of different neuronal subtypes are poorly understood. Here we demonstrate that the bHLH transcription factor Neurogenin2 (Ngn2) is both necessary and sufficient for specifying the dendritic morphology of pyramidal neurons in vivo by specifying the polarity of its leading process during the initiation of radial migration. The ability of Ngn2 to promote a polarized leading process outgrowth requires the phosphorylation of a single tyrosine residue at position 241, an event that is neither involved in Ngn2 direct transactivation properties nor its proneural function. Interestingly, the migration defect observed in the Ngn2 knockout mouse and in progenitors expressing the Ngn2(Y241F) mutation can be rescued by inhibiting the activity of the small-GTPase RhoA in cortical progenitors. Our results demonstrate that Ngn2 coordinates the acquisition of the radial migration properties and the unipolar dendritic morphology characterizing pyramidal neurons through molecular mechanisms distinct from those mediating its proneural activity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix...,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mtap2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neurog2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/beta3 tubulin, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/rhoA GTP-Binding Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0896-6273
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pubmed:author |
pubmed-author:BarnesAnthony PAP,
pubmed-author:BortoneDanteD,
pubmed-author:BouttElizabethE,
pubmed-author:GuerrierSabriceS,
pubmed-author:GuillemotFrançoisF,
pubmed-author:HandRandalR,
pubmed-author:HengJulian Ik-TsenJI,
pubmed-author:MattarPierreP,
pubmed-author:NguyenLaurentL,
pubmed-author:ParrasCarlosC,
pubmed-author:PetersEldonE,
pubmed-author:PolleuxFranckF,
pubmed-author:SchuurmansCarolC
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
45-62
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16202708-Age Factors,
pubmed-meshheading:16202708-Animals,
pubmed-meshheading:16202708-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:16202708-Blotting, Western,
pubmed-meshheading:16202708-Cell Count,
pubmed-meshheading:16202708-Cell Movement,
pubmed-meshheading:16202708-Cells, Cultured,
pubmed-meshheading:16202708-Chickens,
pubmed-meshheading:16202708-Cloning, Molecular,
pubmed-meshheading:16202708-Dendrites,
pubmed-meshheading:16202708-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:16202708-Electroporation,
pubmed-meshheading:16202708-Embryo, Mammalian,
pubmed-meshheading:16202708-Embryo, Nonmammalian,
pubmed-meshheading:16202708-Female,
pubmed-meshheading:16202708-Fluorescent Antibody Technique,
pubmed-meshheading:16202708-Gene Expression Regulation, Developmental,
pubmed-meshheading:16202708-Green Fluorescent Proteins,
pubmed-meshheading:16202708-Humans,
pubmed-meshheading:16202708-Male,
pubmed-meshheading:16202708-Mice,
pubmed-meshheading:16202708-Microscopy, Confocal,
pubmed-meshheading:16202708-Microtubule-Associated Proteins,
pubmed-meshheading:16202708-Models, Biological,
pubmed-meshheading:16202708-Neocortex,
pubmed-meshheading:16202708-Nerve Tissue Proteins,
pubmed-meshheading:16202708-Phosphorylation,
pubmed-meshheading:16202708-Pregnancy,
pubmed-meshheading:16202708-Pyramidal Cells,
pubmed-meshheading:16202708-Sequence Alignment,
pubmed-meshheading:16202708-Stem Cells,
pubmed-meshheading:16202708-Time Factors,
pubmed-meshheading:16202708-Tubulin,
pubmed-meshheading:16202708-Tyrosine,
pubmed-meshheading:16202708-rhoA GTP-Binding Protein
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pubmed:year |
2005
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pubmed:articleTitle |
Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex.
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pubmed:affiliation |
Department of Phamacology, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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