Linked Life Data

16202616

Source:http://linkedlifedata.com/resource/pubmed/id/16202616

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-2-28
pubmed:abstractText
The aim of this study was to investigate the role of microglia in radiation-induced astrocyte gliosis. We found that a single dose of 15 Gy radiation to a whole rat brain increased immunostaining of glial fibrillary acidic protein in astrocytes 6 h later, and even more so 24 h later, indicating the initiation of gliosis. While irradiation of cultured rat astrocytes had little effect, irradiation of microglia-astrocyte mixed-cultures displayed altered astrocyte phenotype into more processed, which is another characteristic of gliosis. Experiments using microglia-conditioned media indicated this astrocyte change was due to factors released from irradiated microglia. Irradiation of cultured mouse microglial cells induced a dose-dependent increase in mRNA levels for cyclooxygenase-2 (COX-2), interleukin (IL)-1beta, IL-6, IL-18, tumor necrosis factor-alpha and interferon-gamma-inducible protein-10, which are usually associated with microglia activation. Consistent with these findings, irradiation of microglia activated NF-kappaB, a transcription factor that regulates microglial activation. Addition of prostaglandin E2 (PGE2: a metabolic product of the COX-2 enzyme) to primary cultured rat astrocytes resulted in phenotypic changes similar to those observed in mixed-culture experiments. Therefore, it appears that PGE(2) released from irradiated microglia is a key mediator of irradiation-induced gliosis or astrocyte phenotype change. These data suggest that radiation-induced microglial activation and resultant production of PGE2 seems to be associated with an underlying cause of inflammatory complications associated with radiation therapy for malignant gliomas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0969-9961
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
457-67
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16202616-Animals, pubmed-meshheading:16202616-Astrocytes, pubmed-meshheading:16202616-Cells, Cultured, pubmed-meshheading:16202616-Coculture Techniques, pubmed-meshheading:16202616-Culture Media, Conditioned, pubmed-meshheading:16202616-Cyclooxygenase 2, pubmed-meshheading:16202616-Cyclooxygenase Inhibitors, pubmed-meshheading:16202616-Dinoprostone, pubmed-meshheading:16202616-Electrophoretic Mobility Shift Assay, pubmed-meshheading:16202616-Glial Fibrillary Acidic Protein, pubmed-meshheading:16202616-Gliosis, pubmed-meshheading:16202616-Immunoblotting, pubmed-meshheading:16202616-Immunohistochemistry, pubmed-meshheading:16202616-Mice, pubmed-meshheading:16202616-Microglia, pubmed-meshheading:16202616-Nitrobenzenes, pubmed-meshheading:16202616-RNA, Messenger, pubmed-meshheading:16202616-Radiation, Ionizing, pubmed-meshheading:16202616-Rats, pubmed-meshheading:16202616-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16202616-Sulfonamides
pubmed:year
2006
pubmed:articleTitle
Ionizing radiation induces astrocyte gliosis through microglia activation.
pubmed:affiliation
Research Institute, National Cancer Center, Goyang, Gyeonggi, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't