Linked Life Data

16199102

Source:http://linkedlifedata.com/resource/pubmed/id/16199102

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-10-18
pubmed:abstractText
alpha1,3/4-Fucosyltransferase (FUT3) is involved in the synthesis of sialyl Le(a) tetrasaccharide, a tumor-associated carbohydrate antigen. Fucosyltransferases are thought to be important regulatory enzymes in the synthesis of fucosylated structures. However, there are conflicting data on the role of FUT3 in the synthesis of this carbohydrate structure and more studies on the regulation of FUT III gene expression are needed. Therefore, as first step, the promoter of FUT III gene was cloned and characterized. Sequencing data showed the absence of TATA, CAAT, and GC boxes, but many binding sites for transcription factors, previously described in colon cancer cells, were identified. Analysis of enhancer and silencing elements of deletion mutants revealed the presence of basal promoter elements of the FUT III gene in the region -636 to -674 bp from the translation initiation site, and positive and negative regulatory elements within the -674 bp to -854 bp and -854 to -1220 regions, respectively. 5'-RACE analysis showed the presence of two transcripts with 5'-ends localized within the exon A. The 5'-end of the longer transcript extended -229 nucleotides from the translation start codon and contained a sequence corresponding to an Inr element, localizing the putative transcription initiation site within this sequence. The strong correlation between the promoter activity of the FUT III gene and the high expression of sialyl Le(a) observed in different colon carcinoma cell lines seem to confirm the important regulatory role of FUT3 in the synthesis of sialyl Le(a).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
1731
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
66-73
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Promoter analysis of the human alpha1,3/4-fucosyltransferase gene (FUT III).
pubmed:affiliation
Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Science, Weigla Str. 12, 53-114 Wroc?aw, Poland.
pubmed:publicationType
Journal Article