16198268

Source:http://linkedlifedata.com/resource/pubmed/id/16198268

Download in:

Switch to

Custom View

Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0032520, umls-concept:C0872367, umls-concept:C1521871
pubmed:issue	2
pubmed:dateCreated	2005-10-3
pubmed:abstractText	The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/100888800
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA
pubmed:status	MEDLINE
pubmed:month	Aug
pubmed:issn	1389-1723
pubmed:author	pubmed-author:KatsuraShinjiS, pubmed-author:KomatsuJunJ, pubmed-author:KuritaHirofumiH, pubmed-author:MizunoAkiraA, pubmed-author:NakanoMichihikoM, pubmed-author:YasudaHachiroH
pubmed:issnType	Print
pubmed:volume	100
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	216-8
pubmed:dateRevised	2006-11-15
pubmed:meshHeading	pubmed-meshheading:16198268-Base Sequence, pubmed-meshheading:16198268-DNA, pubmed-meshheading:16198268-Plasmids, pubmed-meshheading:16198268-Polymerase Chain Reaction
pubmed:year	2005
pubmed:articleTitle	Adaptor polymerase chain reaction for single molecule amplification.
pubmed:affiliation	Department of Ecological Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't