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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1992-8-6
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pubmed:abstractText |
To investigate the role of Ca2+ metabolism and pH in diabetic cardiomyopathy, intracellular Ca2+ concentration ([Ca2+]i) and intracellular pH (pHi) of isolated myocytes were measured simultaneously using fura-2 and BCECF. We used diabetic (D.M.) rats at 8 weeks after the injection of streptozotocin (45 mg/kg, i.v.). (1) [Ca2+]i of D.M. myocytes was lower than that of controls (53 +/- 3 and 75 +/- 5 nM, mean +/- S.E., P less than 0.01). There was no difference in pHi (7.06 +/- 0.02 in D. M., 7.07 +/- 0.02 in control). There was no difference in the percentage of non-rounded cells at 30 min after the perfusion of glucose-free solution which contained 2 mM sodium cyanide (NaCN) between D.M. and controls (53% and 52%). When cells were rounded, the value of [Ca2+]i was significantly lower in D.M. myocytes than that in controls (172 +/- 21 and 421 +/- 106 nM, P less than 0.05). (2) When the cells were shortened or rounded in the high [Ca2+]o solution (24.5 mM), [Ca2+]i of D.M. rats was significantly lower than that of control rats. (3) The percentage of non-rounded cells at 30 min after the perfusion of NaCN increased in controls by 50 mM glucose (95%, P less than 0.01), but not in D.M. (47%). Insulin (25 mU/ml) and glucose (15 mM) increased the percentage of non-rounded cells in D.M. after 30 min perfusion with NaCN (88%, P less than 0.01 v.s. 53% without glucose nor insulin). It is suggested that there are disturbances of Ca2+ metabolism in D.M. myocytes, and that there is a close relation between cell injury and glucose utilization during metabolic inhibition.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-2828
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
435-46
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1619671-Animals,
pubmed-meshheading:1619671-Calcium,
pubmed-meshheading:1619671-Cardiomyopathies,
pubmed-meshheading:1619671-Cytosol,
pubmed-meshheading:1619671-Diabetes Mellitus, Experimental,
pubmed-meshheading:1619671-Energy Metabolism,
pubmed-meshheading:1619671-Glucose,
pubmed-meshheading:1619671-Hydrogen-Ion Concentration,
pubmed-meshheading:1619671-Insulin,
pubmed-meshheading:1619671-Male,
pubmed-meshheading:1619671-Myocardium,
pubmed-meshheading:1619671-Rats,
pubmed-meshheading:1619671-Rats, Inbred Strains,
pubmed-meshheading:1619671-Sodium Cyanide
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pubmed:year |
1992
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pubmed:articleTitle |
Cytosolic Ca2+ concentration and pH of diabetic rat myocytes during metabolic inhibition.
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pubmed:affiliation |
Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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