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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2005-12-1
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pubmed:abstractText |
The junctional adhesion molecule-C (JAM-C) was recently shown to be a counter receptor for the leukocyte beta2-integrin Mac-1 (CD11b/CD18), thereby mediating interactions between vascular cells, particularly in inflammatory cell recruitment. Here, we investigated the role of JAM-C in oxidized low-density lipoprotein (LDL)-mediated leukocyte recruitment. As compared with normal arteries, immunostaining of atherosclerotic vessels revealed a high expression of JAM-C in association with neointimal smooth muscle cells and the endothelium. Moreover, JAM-C was strongly up-regulated in the spontaneous early lesions in ApoE -/- mice. In vitro, cultured human arterial smooth muscle cells (HASMC) were found to express JAM-C, and oxLDL, as well as enzymatically modified LDL (eLDL) significantly up-regulated JAM-C on both HASMC and endothelial cells in a time- and dose-dependent manner. Although under quiescent conditions, JAM-C predominantly localized to interendothelial cell-cell contacts in close proximity to zonula occludens-1 (ZO-1), oxLDL treatment induced a disorganization of JAM-C localization that was no more restricted to the interendothelial junctions. JAM-C thereby mediated both leukocyte adhesion and leukocyte transendothelial migration upon oxLDL treatment of endothelial cells, whereas JAM-C on quiescent endothelial cells only mediates leukocyte transmigration. Thus, upon oxLDL stimulation endothelial JAM-C functions as both an adhesion, as well as a transmigration receptor for leukocytes. Taken together, JAM-C is up-regulated by oxLDL and may thereby contribute to increased inflammatory cell recruitment during atherosclerosis. JAM-C may therefore provide a novel molecular target for antagonizing interactions between vascular cells in atherosclerosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD11b,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/JAM3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1530-6860
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pubmed:author |
pubmed-author:Al-FakhriNadiaN,
pubmed-author:AthanasopoulosAthanasios NAN,
pubmed-author:BohleRainer MRM,
pubmed-author:ChavakisEmmanouilE,
pubmed-author:ChavakisTriantafyllosT,
pubmed-author:HaendelerJudithJ,
pubmed-author:HersemeyerKarinK,
pubmed-author:HerzogStefanieS,
pubmed-author:IsermannBerendB,
pubmed-author:KeiperTanjaT,
pubmed-author:PreissnerKlaus TKT,
pubmed-author:SaffrichRainerR,
pubmed-author:SantosoSentotS
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pubmed:issnType |
Electronic
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2078-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16195363-Aged,
pubmed-meshheading:16195363-Animals,
pubmed-meshheading:16195363-Antigens, CD11b,
pubmed-meshheading:16195363-Antigens, CD18,
pubmed-meshheading:16195363-Aorta,
pubmed-meshheading:16195363-Atherosclerosis,
pubmed-meshheading:16195363-Blotting, Western,
pubmed-meshheading:16195363-Cell Adhesion,
pubmed-meshheading:16195363-Cell Adhesion Molecules,
pubmed-meshheading:16195363-Cell Movement,
pubmed-meshheading:16195363-DNA, Complementary,
pubmed-meshheading:16195363-Dose-Response Relationship, Drug,
pubmed-meshheading:16195363-Endothelial Cells,
pubmed-meshheading:16195363-Endothelium, Vascular,
pubmed-meshheading:16195363-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16195363-Female,
pubmed-meshheading:16195363-Humans,
pubmed-meshheading:16195363-Immunoglobulin G,
pubmed-meshheading:16195363-Immunoglobulins,
pubmed-meshheading:16195363-Immunohistochemistry,
pubmed-meshheading:16195363-Inflammation,
pubmed-meshheading:16195363-Leukocytes,
pubmed-meshheading:16195363-Lipoproteins, LDL,
pubmed-meshheading:16195363-Male,
pubmed-meshheading:16195363-Membrane Proteins,
pubmed-meshheading:16195363-Mice,
pubmed-meshheading:16195363-Mice, Transgenic,
pubmed-meshheading:16195363-Microscopy, Fluorescence,
pubmed-meshheading:16195363-Middle Aged,
pubmed-meshheading:16195363-Models, Biological,
pubmed-meshheading:16195363-Monocytes,
pubmed-meshheading:16195363-Muscle, Smooth, Vascular,
pubmed-meshheading:16195363-Myocytes, Smooth Muscle,
pubmed-meshheading:16195363-Oxygen,
pubmed-meshheading:16195363-Recombinant Fusion Proteins,
pubmed-meshheading:16195363-Recombinant Proteins,
pubmed-meshheading:16195363-Time Factors,
pubmed-meshheading:16195363-Umbilical Veins,
pubmed-meshheading:16195363-Up-Regulation
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pubmed:year |
2005
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pubmed:articleTitle |
The role of junctional adhesion molecule-C (JAM-C) in oxidized LDL-mediated leukocyte recruitment.
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pubmed:affiliation |
Experimental Immunology Branch, NCI, NIH, Bethesda, Maryland 20892, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Intramural
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