Linked Life Data

16189269

Source:http://linkedlifedata.com/resource/pubmed/id/16189269

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-3-6
pubmed:abstractText
Platelet-derived growth factor D-chain (PDGF-D) is the newest member of the PDGF family of mitogens and chemoattractants expressed in a wide variety of cell types, including vascular smooth muscle cells (SMCs). The molecular mechanisms regulating PDGF-D transcription are not known. Primer extension analysis mapped a single transcriptional start site to the ccAGCGC motif with several potential Ets motifs located upstream. Ets-1, but not Ets-1 bearing only the DNA-binding domain, activates the PDGF-D promoter and mRNA expression in SMCs. Ets site D3 ((-470)GGAT(-467)) is singly required for basal and Ets-1-inducible PDGF-D promoter-dependent expression. D3 supports the interaction of endogenous and recombinant Ets-1 and Sp1. Sp1, like Ets-1, induces PDGF-D transcription and mRNA expression, which is blocked by mutant Ets-1. H2O2 stimulates Ets-1, but not Sp1, and activates D3-dependent PDGF-D transcription. Ets-1 and Sp1 siRNA block peroxide-inducible PDGF-D expression. Angiotensin II (ATII) induction of PDGF-D and Ets-1 was blocked by prior incubation of the cells with PEG-catalase, but not BSA, indicating that ATII-inducible Ets-1 and PDGF-D expression is mediated via H2O2. Thus, 2 separate trans-acting factors regulate PDGF-D transcription, alone and in response to oxidative stress.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2322-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16189269-Angiotensin II, pubmed-meshheading:16189269-Animals, pubmed-meshheading:16189269-Cattle, pubmed-meshheading:16189269-Cells, Cultured, pubmed-meshheading:16189269-Gene Expression Regulation, pubmed-meshheading:16189269-Humans, pubmed-meshheading:16189269-Hydrogen Peroxide, pubmed-meshheading:16189269-Lymphokines, pubmed-meshheading:16189269-Muscle, Smooth, Vascular, pubmed-meshheading:16189269-Oxidative Stress, pubmed-meshheading:16189269-Platelet-Derived Growth Factor, pubmed-meshheading:16189269-Promoter Regions, Genetic, pubmed-meshheading:16189269-Proto-Oncogene Protein c-ets-1, pubmed-meshheading:16189269-Rats, pubmed-meshheading:16189269-Sp1 Transcription Factor, pubmed-meshheading:16189269-Transcription, Genetic, pubmed-meshheading:16189269-Transcription Initiation Site
pubmed:year
2006
pubmed:articleTitle
Inducible platelet-derived growth factor D-chain expression by angiotensin II and hydrogen peroxide involves transcriptional regulation by Ets-1 and Sp1.
pubmed:affiliation
Centre for Vascular Research, Department of Pathology, University of New South Wales, Sydney NSW 2052 Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't