Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
19
|
pubmed:dateCreated |
1992-8-5
|
pubmed:abstractText |
Levels of expression of the platelet-derived growth factor (PDGF) A-chain gene differ significantly in normal and transformed cells. We have identified an S1 nuclease-sensitive site in a GC box located at -55 to -72 in the PDGF A-chain promoter region. We now demonstrate that a 24-base, G-rich complementary oligonucleotide anneals specifically to the C-rich strand of the GC box and protects the GC box from nicking by S1 nuclease whereas the C-rich complementary oligonucleotide and its double-stranded counterpart do not. In transient transfection assays, expression of the PDGF A-chain gene is sharply reduced by deletions within the GC box or if the 24-base G-rich complementary oligonucleotide is preincubated with the promoter construct prior to transfection. The data suggest that the GC box of the PDGF A-chain gene may promote a non-B-form DNA structure, which is recognized by S1 nuclease and which anneals to a short complementary G-rich oligonucleotide. The data also suggest that this non-B-form DNA is important for efficient transcription of PDGF A-chain gene.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0021-9258
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
5
|
pubmed:volume |
267
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
13669-74
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:1618866-Animals,
pubmed-meshheading:1618866-Autoradiography,
pubmed-meshheading:1618866-Base Sequence,
pubmed-meshheading:1618866-Binding Sites,
pubmed-meshheading:1618866-Cattle,
pubmed-meshheading:1618866-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1618866-DNA,
pubmed-meshheading:1618866-HeLa Cells,
pubmed-meshheading:1618866-Humans,
pubmed-meshheading:1618866-Molecular Sequence Data,
pubmed-meshheading:1618866-Oligonucleotides,
pubmed-meshheading:1618866-Plasmids,
pubmed-meshheading:1618866-Platelet-Derived Growth Factor,
pubmed-meshheading:1618866-Promoter Regions, Genetic,
pubmed-meshheading:1618866-Transcription, Genetic,
pubmed-meshheading:1618866-Transfection
|
pubmed:year |
1992
|
pubmed:articleTitle |
Binding of single-stranded oligonucleotides to a non-B-form DNA structure results in loss of promoter activity of the platelet-derived growth factor A-chain gene.
|
pubmed:affiliation |
Department of Medicine, Washington University Medical Center, St. Louis, Missouri.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|