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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1992-8-6
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pubmed:databankReference |
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pubmed:abstractText |
The human renin-binding protein (RnBP) gene was isolated from a human placental genomic library and characterized. The gene spans about 10 kilobases and consists of 11 exons separated by 10 introns. The 5'-flanking region and the exon-intron boundaries were sequenced. Residue G* in 5'-CGAG*TGG-3' was identified as the major transcription initiation site, and "GC" boxes were found in the vicinity of the cap site. No typical "TATA" or "CCAAT" box exists in the 5'-flanking region. The hydrophobic domain followed by a leucine-zipper motif in RnBP is encoded by the sixth exon. A fragment of the human RnBP gene (nucleotides -739 to +244) linked to the chloramphenicol acetyltransferase gene was transfected into human Wilms' tumor G401 and mouse L929 cells. The expression of this chimeric gene in G401 cells was 4-fold higher than that in L929 cells, the tissue-specific regulation of RnBP gene expression thus being suggested. The promoter for the RnBP gene was shown to be localized in nucleotides -35 to +244 on assaying of the promoter activity using deletion mutants of the chimeric constructs. The RnBP gene was found to be located in human chromosome X by means of polymerase chain reaction of hybrid DNAs from human and hamster somatic cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
267
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pubmed:geneSymbol |
RnBP
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13007-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1618798-Animals,
pubmed-meshheading:1618798-Base Sequence,
pubmed-meshheading:1618798-Carbohydrate Epimerases,
pubmed-meshheading:1618798-Carrier Proteins,
pubmed-meshheading:1618798-Cell Line,
pubmed-meshheading:1618798-Chromosome Mapping,
pubmed-meshheading:1618798-DNA,
pubmed-meshheading:1618798-Exons,
pubmed-meshheading:1618798-Humans,
pubmed-meshheading:1618798-Introns,
pubmed-meshheading:1618798-Mice,
pubmed-meshheading:1618798-Molecular Sequence Data,
pubmed-meshheading:1618798-Renin,
pubmed-meshheading:1618798-Restriction Mapping,
pubmed-meshheading:1618798-Transcription, Genetic,
pubmed-meshheading:1618798-X Chromosome
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pubmed:year |
1992
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pubmed:articleTitle |
The human gene for renin-binding protein.
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pubmed:affiliation |
Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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