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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2005-9-27
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pubmed:abstractText |
BETA2/NeuroD, a basic helix-loop-helix transcription factor, is a key regulator of pancreatic islet morphogenesis and insulin gene transcription. Here we report for the first time that the BETA2/NeuroD protein can permeate several cells, including pancreatic islets, due to an arginine- and lysine-rich protein transduction domain sequence in its structure. The BETA2/NeuroD protein was transduced in a dose-dependent manner up to 1 micromol/l. Transduced BETA2/NeuroD functions similarly to endogenous BETA2/NeuroD: it binds to the insulin promoter and activates its expression. We also investigated the mechanism of BETA2/NeuroD protein transduction. The BETA2/NeuroD protein penetrated cells by macropinocytosis and was released from endosomes homogeneously in cytoplasm and nuclei. These data suggest that BETA2/NeuroD protein transduction could be a safe and valuable strategy for enhancing insulin gene transcription without requiring gene transfer technology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0012-1797
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2859-66
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pubmed:dateRevised |
2006-4-21
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pubmed:meshHeading |
pubmed-meshheading:16186386-Amino Acid Sequence,
pubmed-meshheading:16186386-Animals,
pubmed-meshheading:16186386-Arginine,
pubmed-meshheading:16186386-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:16186386-Cells, Cultured,
pubmed-meshheading:16186386-Cloning, Molecular,
pubmed-meshheading:16186386-Endocytosis,
pubmed-meshheading:16186386-Genetic Vectors,
pubmed-meshheading:16186386-Green Fluorescent Proteins,
pubmed-meshheading:16186386-HeLa Cells,
pubmed-meshheading:16186386-Humans,
pubmed-meshheading:16186386-Islets of Langerhans,
pubmed-meshheading:16186386-Liver,
pubmed-meshheading:16186386-Lysine,
pubmed-meshheading:16186386-Male,
pubmed-meshheading:16186386-Membrane Microdomains,
pubmed-meshheading:16186386-Molecular Sequence Data,
pubmed-meshheading:16186386-Phagocytosis,
pubmed-meshheading:16186386-Pinocytosis,
pubmed-meshheading:16186386-Protein Transport,
pubmed-meshheading:16186386-Rats,
pubmed-meshheading:16186386-Rats, Sprague-Dawley,
pubmed-meshheading:16186386-Recombinant Fusion Proteins,
pubmed-meshheading:16186386-Recombinant Proteins,
pubmed-meshheading:16186386-Structure-Activity Relationship,
pubmed-meshheading:16186386-Transfection
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pubmed:year |
2005
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pubmed:articleTitle |
BETA2/NeuroD protein can be transduced into cells due to an arginine- and lysine-rich sequence.
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pubmed:affiliation |
Dept. of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507, Japan. noguchih@kuhp.kyoto-u.ac.jp
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pubmed:publicationType |
Journal Article
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