Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-9-27
pubmed:abstractText
The GTPases Rac and Cdc42 play a pivotal role in the establishment of cell polarity by stimulating biogenesis of tight junctions (TJs). In this study, we show that the Rac-specific guanine nucleotide exchange factor Tiam1 (T-lymphoma invasion and metastasis) controls the cell polarity of epidermal keratinocytes. Similar to wild-type (WT) keratinocytes, Tiam1-deficient cells establish primordial E-cadherin-based adhesions, but subsequent junction maturation and membrane sealing are severely impaired. Tiam1 and V12Rac1 can rescue the TJ maturation defect in Tiam1-deficient cells, indicating that this defect is the result of impaired Tiam1-Rac signaling. Tiam1 interacts with Par3 and aPKCzeta, which are two components of the conserved Par3-Par6-aPKC polarity complex, and triggers biogenesis of the TJ through the activation of Rac and aPKCzeta, which is independent of Cdc42. Rac is activated upon the formation of primordial adhesions (PAs) in WT but not in Tiam1-deficient cells. Our data indicate that Tiam1-mediated activation of Rac in PAs controls TJ biogenesis and polarity in epithelial cells by association with and activation of the Par3-Par6-aPKC polarity complex.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-10852816, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-10934475, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-11036612, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-11163137, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-11283726, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-11489913, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-11839275, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-12075356, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-12186943, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-12507993, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-12717816, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-12829230, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-12901789, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-14530286, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-15027470, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-15138270, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-15286792, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-15479739, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-15677455, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-15723051, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-15723052, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-15775979, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-9015310, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-9660866, http://linkedlifedata.com/resource/pubmed/commentcorrection/16186252-9832565
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1029-37
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
The Rac activator Tiam1 controls tight junction biogenesis in keratinocytes through binding to and activation of the Par polarity complex.
pubmed:affiliation
Division of Cell Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't