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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-11-18
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pubmed:abstractText |
Downregulation of MHC class I molecules is a strategy developed by some viruses to escape cellular immune responses. Myxoma virus (MV), a poxvirus causing rabbit myxomatosis, encodes MV-LAP that is known to increase MHC-I endocytosis and degradation through a C(4)HC(3) motif critical for an E3 ubiquitin ligase activity. Here, we performed a functional mapping of MV-LAP and showed that not only the C(4)HC(3) motif is necessary for a marked downregulation of MHC-I but also a conserved region in the C-terminal part of the protein. We also showed that the putative transmembrane domains are responsible for a specific subcellular localization of the protein: they retain MV-LAP in the ER in transfected cells and in the endolysosomal compartments in infected cells. We observed that a specific MV infection context is necessary for a fully efficient downregulation of MHC-I. Our data suggest that the functionality of viral LAP factors, inherited by herpes- and poxviruses from mammalian cells, is more complex than anticipated.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
343
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16185739-Amino Acid Sequence,
pubmed-meshheading:16185739-Animals,
pubmed-meshheading:16185739-Base Sequence,
pubmed-meshheading:16185739-Cell Line,
pubmed-meshheading:16185739-Conserved Sequence,
pubmed-meshheading:16185739-DNA, Viral,
pubmed-meshheading:16185739-Down-Regulation,
pubmed-meshheading:16185739-Genes, Viral,
pubmed-meshheading:16185739-Histocompatibility Antigens Class I,
pubmed-meshheading:16185739-Membrane Proteins,
pubmed-meshheading:16185739-Molecular Sequence Data,
pubmed-meshheading:16185739-Mutation,
pubmed-meshheading:16185739-Myxoma virus,
pubmed-meshheading:16185739-Myxomatosis, Infectious,
pubmed-meshheading:16185739-Peptide Mapping,
pubmed-meshheading:16185739-Rabbits,
pubmed-meshheading:16185739-Recombinant Fusion Proteins,
pubmed-meshheading:16185739-Sequence Homology, Amino Acid,
pubmed-meshheading:16185739-Transfection,
pubmed-meshheading:16185739-Viral Proteins
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pubmed:year |
2005
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pubmed:articleTitle |
The poxviral scrapin MV-LAP requires a myxoma viral infection context to efficiently downregulate MHC-I molecules.
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pubmed:affiliation |
UMR 1225 Interactions Hôtes Agents pathogènes, INRA/ENVT, Ecole Nationale Vétérinaire de Toulouse, 23 chemin des Capelles, BP87614, 31076 Toulouse cedex 03, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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