Source:http://linkedlifedata.com/resource/pubmed/id/16185276
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2005-9-27
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pubmed:abstractText |
The local cytokine environment and the presence of stimulatory signals determine whether monocytes acquire dendritic cell or macrophage characteristics and functions. In this study, we examined the effect of histamine, a prototypic mediator of allergic inflammation, on the granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4-driven differentiation of monocytes into monocyte-derived dendritic cells (MoDC), which typically showed CD1a+CD14- phenotype. Monocytes from healthy adult donors were cultured with GM-CSF and IL-4 in the presence or absence of histamine, and the phenotypes and function of these cells were analyzed. Histamine induced the generation of CD1a-CD14+ cells, which exhibited cytological and phenotypical characteristics of dendritic cells (DC), showed enhanced phagocytic activity and cytokine-producing capacity, but demonstrated weak allo-stimulatory capacity compared with CD1a+CD14- MoDC. The inhibitory effects of histamine on CD1a+CD14- MoDC differentiation were antagonized by cimetidine, an H2 receptor antagonist, but not by H1 and H3 receptor blockers, and were mimicked by an H2 receptor agonist. Culture supernatant of histamine-treated monocytes also inhibited CD1a+CD14- MoDC differentiation, which was restored by the removal of IL-10. These results suggest that histamine-driven CD1a-CD14+ DC amplify their antigen-independent inflammatory reaction and may contribute to the exacerbation of allergic diseases.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/CD1a antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Histamine H2
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-202X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
753-60
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16185276-Antigens, CD1,
pubmed-meshheading:16185276-Antigens, CD14,
pubmed-meshheading:16185276-Cell Differentiation,
pubmed-meshheading:16185276-Cells, Cultured,
pubmed-meshheading:16185276-Cytokines,
pubmed-meshheading:16185276-Dendritic Cells,
pubmed-meshheading:16185276-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:16185276-Histamine,
pubmed-meshheading:16185276-Humans,
pubmed-meshheading:16185276-Interleukin-4,
pubmed-meshheading:16185276-Monocytes,
pubmed-meshheading:16185276-Receptors, Histamine H2
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pubmed:year |
2005
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pubmed:articleTitle |
Histamine induces the generation of monocyte-derived dendritic cells that express CD14 but not CD1a.
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pubmed:affiliation |
Department of Dermatology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan. nkatoh@koto.kpu-m.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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