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rdf:type |
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lifeskim:mentions |
umls-concept:C0004083,
umls-concept:C0007634,
umls-concept:C0010453,
umls-concept:C0012854,
umls-concept:C0013227,
umls-concept:C0017262,
umls-concept:C0026724,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0069562,
umls-concept:C0162638,
umls-concept:C0185117,
umls-concept:C0237477,
umls-concept:C1704242,
umls-concept:C2003941,
umls-concept:C2249823,
umls-concept:C2911684
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pubmed:dateCreated |
2005-9-26
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pubmed:abstractText |
Rebamipide, a gastromucosal protective drug, suppresses indomethacin-induced gastropathy in humans and rodents. Effects of rebamipide on gene expression in indomethacin-treated gastric mucosal cells (RGM1) were investigated using high-density oligonucleotide arrays. Indomethacin induced apoptosis in RGM1 cells in a dose-dependent manner. Rebamipide pretreatment significantly reduced indomethacin-induced apoptosis. We used gene expression profiling on high-density oligonucleotide probe arrays to characterize the transcriptional response of RGM1 cells to indomethacin treatment for 6 hr. Of the 8,799 probes examined, 717 (8.1%) were induced (400 probes) or repressed (317 probes) at least 1.5-fold. Among the 158 genes that were induced by indomethacin at least 2.0-fold, four genes that were down-regulated by rebamipide at least 2.0-fold are listed: growth arrest and DNA-damage-inducible 45 alpha (GADD 45 alpha), golgi SNAP receptor complex member 1, iodothyronine deiodinases, and transcription factor 8. Real time-PCR confirmed GADD 45 alpha expression and its inhibition by rebamipide. Inhibition of apoptosis-related genes is possibly important for the cytoprotective effect of rebamipide against indomethacin-induced gastric mucosal cell injury.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Ulcer Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Gadd45a protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Quinolones,
http://linkedlifedata.com/resource/pubmed/chemical/rebamipide
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0163-2116
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pubmed:author |
pubmed-author:HandaOsamuO,
pubmed-author:IchikawaHiroshiH,
pubmed-author:KajikawaHirokazuH,
pubmed-author:KatadaKazuhiroK,
pubmed-author:KokuraSatoshiS,
pubmed-author:KurodaMasaakiM,
pubmed-author:MatsuiHirofumiH,
pubmed-author:MizushimaKatsuraK,
pubmed-author:NaitoYujiY,
pubmed-author:ShimozawaMakotoM,
pubmed-author:TakagiTomohisaT,
pubmed-author:YoshidaNorimasaN,
pubmed-author:YoshikawaToshikazuT
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pubmed:issnType |
Print
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pubmed:volume |
50 Suppl 1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
S104-12
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:16184411-Alanine,
pubmed-meshheading:16184411-Animals,
pubmed-meshheading:16184411-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:16184411-Anti-Ulcer Agents,
pubmed-meshheading:16184411-Apoptosis,
pubmed-meshheading:16184411-Cell Culture Techniques,
pubmed-meshheading:16184411-Cell Cycle Proteins,
pubmed-meshheading:16184411-DNA Damage,
pubmed-meshheading:16184411-Dose-Response Relationship, Drug,
pubmed-meshheading:16184411-Down-Regulation,
pubmed-meshheading:16184411-Gastric Mucosa,
pubmed-meshheading:16184411-Gene Expression Profiling,
pubmed-meshheading:16184411-Indomethacin,
pubmed-meshheading:16184411-Nuclear Proteins,
pubmed-meshheading:16184411-Oligonucleotide Probes,
pubmed-meshheading:16184411-Polymerase Chain Reaction,
pubmed-meshheading:16184411-Quinolones,
pubmed-meshheading:16184411-Rats
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pubmed:year |
2005
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pubmed:articleTitle |
Rebamipide, a gastro-protective drug, inhibits indomethacin-induced apoptosis in cultured rat gastric mucosal cells: association with the inhibition of growth arrest and DNA damage-induced 45 alpha expression.
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pubmed:affiliation |
Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. ynaito@koto.kpu-m.ac.jp
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pubmed:publicationType |
Journal Article
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