Linked Life Data

16183038

Source:http://linkedlifedata.com/resource/pubmed/id/16183038

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2005-10-24
pubmed:abstractText
Bufalin, a bufadienolide type cardiotonic steroid that is one of the major components of the toad venom-prepared traditional Chinese medicine called Ch'an Su or Senso, exhibits a cardiotonic action by inhibiting the membranous Na(+),K(+)-ATPase. Bufalin also induces differentiation of leukemia cells alone or in combination with other differentiation inducers including 1alpha,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)]. In this study, we performed a transient cotransfection assay using a vitamin D receptor (VDR) expression vector and a luciferase reporter and found that although bufalin did not transactivate the VDR, it effectively enhanced VDR activity induced by 1,25(OH)(2)D(3). Bufalin also augmented VDR activation by bile acid ligands, such as lithocholic acid and 3-ketocholanic acid. Other cardiotonic steroids including ouabain, digitoxigenin and cinobufagin did not enhance VDR activation. Bufalin did not bind directly to VDR but did modulate the interaction of VDR and cofactors, such as steroid receptor coactivator-1 and nuclear receptor corepressor. Bufalin treatment significantly increased the expression of an endogenous VDR target gene, CYP24, in kidney- and monocyte-derived cell lines treated with 1,25(OH)(2)D(3). The data indicate that bufalin-mediated cellular mechanisms such as interaction with Na(+), K(+)-ATPase may affect VDR transcriptional activity. Bufalin may be a useful tool in the investigation of VDR regulation by membrane-originating cellular signals and of pathophysiological mechanisms linking VDR to cardiovascular dysfunction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1479-86
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16183038-Amphibian Venoms, pubmed-meshheading:16183038-Animals, pubmed-meshheading:16183038-Anura, pubmed-meshheading:16183038-Bufanolides, pubmed-meshheading:16183038-Calcitriol, pubmed-meshheading:16183038-Cardiac Glycosides, pubmed-meshheading:16183038-Cell Line, pubmed-meshheading:16183038-Dose-Response Relationship, Drug, pubmed-meshheading:16183038-Drug Interactions, pubmed-meshheading:16183038-Drug Synergism, pubmed-meshheading:16183038-Drug Therapy, Combination, pubmed-meshheading:16183038-Furylfuramide, pubmed-meshheading:16183038-Gene Expression, pubmed-meshheading:16183038-Humans, pubmed-meshheading:16183038-Isotope Labeling, pubmed-meshheading:16183038-Ligands, pubmed-meshheading:16183038-Luciferases, pubmed-meshheading:16183038-Medicine, Chinese Traditional, pubmed-meshheading:16183038-Receptors, Calcitriol, pubmed-meshheading:16183038-Steroid Hydroxylases, pubmed-meshheading:16183038-Transcriptional Activation
pubmed:year
2005
pubmed:articleTitle
Enhancement of ligand-dependent vitamin D receptor transactivation by the cardiotonic steroid bufalin.
pubmed:affiliation
Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't